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Evaluation of the experimental infection in hamster by isolated parasites of atypical cutaneous lesion and human visceral caused by Leishmania (L.) infantum chagasi in the municipality of Amapala, Valle, Honduras

Grant number: 17/24834-9
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2019
Effective date (End): November 30, 2021
Field of knowledge:Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
Principal researcher:Marcia Dalastra Laurenti
Grantee:Gabriela Venicia Araujo Flores
Home Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

In the Americas, particularly in South America, infection by Leishmania (Leishmania) infantum chagasi cause subclinical manifestations and Visceral Leishmaniasis (VL), which if untreated is potentially fatal. In Honduras, infection by Leishmania (L.) infantum chagasi causes Visceral Leishmaniasis (VL), but has also been reported Non-Ulcerated or Atypical Cutaneous Leishmaniasis (NUCL) caused by this species of parasite. Cases of Non-Ulcerated or Atypical Cutaneous Leishmaniasis has also been reported in other countries in Central America, such as Costa Rica, El Salvador and Nicaragua. In Honduras, there are endemic areas well identified of this non-ulcerated clinical variant, located in the departments of Choluteca and Valle, as well as the southern part of departments Francisco Morazán, La Paz, El Paraíso, Intibucá and Lempira. In order to better understand the pathogenesis of the infection caused by this specie of parasite in America, this study aims to evaluate the evolution of the experimental infection in hamsters, Mesocricetus auratus, caused by Leishmania (L.) infantum chagasi isolated from individuals affected with classical Visceral Leishmaniasis and Non-Ulcerated or Atypical Cutaneous Leishmaniasis in the municipality of Amapala, Valle, Honduras. Hamsters will be infected subcutaneously and intraperitoneally with 10.000.000 promastigotes of Leishmania (L.) infantum chagasi in stationary growth phase in culture. After 30, 60 and 90 days Post Infection (PI) animals will be anesthetized, euthanized and fragments of skin and viscera (spleen, liver and lymph node) will be collected for histopathological evaluation, determination of the parasite load and the cytokines expression. Whole blood will be collected and sera were used for determination of anti-Leishmania antibodies. The identification of the parasite will be done by total genome sequencing of the isolates obtained from individuals with LV and LCNU. Besides the identification of the parasite, it is aimed to determine possible differences that may be related to the determination of the different clinical forms. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SANDOVAL PACHECO, CARMEN M.; ARAUJO FLORES, V, GABRIELA; GONZALEZ, KADIR; DE CASTRO GOMES, CLAUDIA M.; PASSERO, LUIZ F. D.; TOMOKANE, THAISE Y.; SOSA-OCHOA, WILFREDO; ZUNIGA, CONCEPCION; CALZADA, JOSE; SALDANA, AZAEL; CORBETT, CARLOS E. P.; SILVEIRA, FERNANDO T.; LAURENTI, MARCIA D. Macrophage Polarization in the Skin Lesion Caused by Neotropical Species of Leishmania sp. JOURNAL OF IMMUNOLOGY RESEARCH, v. 2021, APR 12 2021. Web of Science Citations: 0.
SANDOVAL, CARMEN; ARAUJO, GABRIELA; SOSA, WILFREDO; AVALOS, SARA; SILVEIRA, FERNANDO; CORBETT, CARLOS; ZUNIGA, CONCEPCION; LAURENTI, MARCIA. In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infection. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 27, FEB 26 2021. Web of Science Citations: 1.
CAMILA A CARDOSO; GABRIELA V ARAUJO; CARMEN M SANDOVAL; PAULA M NOGUEIRA; CONCEPCION ZÚNIGA; WILFREDO H SOSA-OCHOA; MÁRCIA D LAURENTI; RODRIGO P SOARES. Lipophosphoglycans from dermotropic Leishmania infantum are more pro-inflammatory than those from viscerotropic strains. Memórias do Instituto Oswaldo Cruz, v. 115, p. -, 2020. Web of Science Citations: 1.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.