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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infection

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Author(s):
Sandoval, Carmen [1] ; Araujo, Gabriela [1] ; Sosa, Wilfredo [2, 1] ; Avalos, Sara [3] ; Silveira, Fernando [4, 5] ; Corbett, Carlos [1] ; Zuniga, Concepcion [6] ; Laurenti, Marcia [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Med Sch FMUSP, Dept Pathol, Lab Infect Dis Pathol, Sao Paulo, SP - Brazil
[2] Natl Autonomous Univ Honduras, Microbiol Res Inst, Tegucigalpa - Honduras
[3] Natl Autonomous Univ Honduras, Sch Microbiol, Master Program Infect & Zoonot Dis, Tegucigalpa - Honduras
[4] Fed Univ Para, Inst Trop Med, Belem, Para - Brazil
[5] Minist Hlth, Dept Parasitol, Evandro Chagas Inst, Secretariat Hlth Surveillance, Belem, Para - Brazil
[6] School Hosp, Dept Hlth Surveillance, Tegucigalpa - Honduras
Total Affiliations: 6
Document type: Journal article
Source: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 27, FEB 26 2021.
Web of Science Citations: 1
Abstract

Background: Skin lesions of patients affected by non-ulcerated cutaneous leishmaniasis (NUCL) caused by L. (L.) infantum chagasi are characterized by lymphohistiocytic inflammatory infiltrate associated with epithelioid granuloma and scarce parasitism. However, the in situ cellular immune response of these patients is unclear. Therefore, the aim of the present study was to characterize the cellular immune response in the skin lesions of patients affected by NUCL. Methods: Twenty biopsies were processed by immunohistochemistry using primary antibodies to T lymphocytes (CD4, CD8), NK cells, B lymphocytes, macrophages, nitric oxide synthase and interferon-gamma. Results: Immunohistochemistry revealed higher expression of all cellular types and molecules (IFN-gamma, iNOS) in the dermis of diseased skin compared to the skin of healthy individuals (p < 0.05). Morphometric analysis performed in the skin lesions sections showed the predominance of CD8(+) T lymphocytes in the mononuclear infiltrate, followed by macrophages, mostly iNOS(+), a response that could be mediated by IFN-gamma. Conclusion: Our study improves knowledge of the cellular immune response in nonulcerated or atypical cutaneous leishmaniasis caused by L. (L.) infantum chagasi in Central America and pointed to the pivotal participation of CD8(+) T lymphocytes in the host defense mechanisms against the parasite in patients with NUCL. (AU)

FAPESP's process: 17/24834-9 - Evaluation of the experimental infection in hamster by isolated parasites of atypical cutaneous lesion and human visceral caused by Leishmania (L.) infantum chagasi in the municipality of Amapala, Valle, Honduras
Grantee:Gabriela Venicia Araujo Flores
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/50315-0 - Leishmaniasis in Latin America: an advanced perspective on immunopathogenetic factors of cutaneous and visceral infection, immunomodulators of the sandflies vector saliva and immunogenic exo-antigens of Leishmania (L.) infantum chagasi as vaccine candidates
Grantee:Carlos Eduardo Pereira Corbett
Support type: Research Projects - Thematic Grants
FAPESP's process: 18/04698-6 - Non-ulcerated or atipycal Cutaneous Leishmaniasis caused by Leishmania (L.) infantum chagasi in the municipality of Amapala, Valle, Honduras: immunohistopathological characterization of skin lesions with emphasis in the participation of antigen presenting cells
Grantee:Carmen Maria Sandoval Pacheco
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/01154-7 - Non-ulcerated or atypical cutaneous leishmaniasis caused by Leishmania infantum chagasi in Amapala municipality, Valle, Honduras: immune-histological characterization of the skin lesions
Grantee:Carmen Maria Sandoval Pacheco
Support type: Scholarships in Brazil - Master