| Texto completo | |
| Autor(es): |
Pereira, Samuel Santos
[1]
;
Andreata-Santos, Robert
[1]
;
Pereira, Lennon Ramos
[1]
;
Soares, Camila Pereira
[2]
;
Felix, Alvina Clara
[3]
;
Jungmann Cardoso de Andrade, Patricia de Mello
[4]
;
Durigon, Edison Luis
[2]
;
Romano, Camila Malta
[3, 5]
;
de Souza Ferreira, Luis Carlos
[1]
Número total de Autores: 9
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Biomed Sci, Microbiol Dept, Vaccine Dev Lab, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Microbiol Dept, Lab Clin & Mol Virol, Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Med Trop Sao Paulo, Sao Paulo - Brazil
[4] Univ Pernambuco UPE, Gen Pathol, Recife, PE - Brazil
[5] Univ Sao Paulo, Hosp Clin HCFMUSP LIM 52, Fac Med, Sao Paulo - Brazil
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES; v. 112, p. 202-204, SEP 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
Objectives: The aim of this study was to achieve greater specificity of dengue virus (DENV) serological tests based on a recombinant antigen derived from non-structural protein 1 (Delta NS1) with regard to cross-reactive Zika virus (ZIKV) anti-NS1 antibody responses. This is of relevance in endemic regions for the serological discrimination of both DENV and ZIKV, such as Brazil and other tropical countries. Methods: The Delta NS1 proteins were obtained as recombinant antigens and were evaluated as solid-phase-bound antigens in the ELISA test to detect anti-NS1 IgG antibodies. The performance of the Delta NS1-based DENV IgG ELISA was assessed with both mouse and human serum samples previously exposed to DENV or ZIKV. Results: The Delta NS1-based DENV IgG ELISA detected anti-DENV NS1 IgG without cross-reactivity with ZIKV-positive serum samples. The sensitivity and specificity of the assay determined using samples previously characterized by real-time PCR (qRT-PCR) or plaque reduction neutralization assay (PRNT) were 82% and 93%, respectively. Conclusion: The Delta NS1-based DENV IgG ELISA conferred enhanced diagnostic specificity for anti-DENV serological tests and may be particularly useful for serological analyses in endemic regions for both DENV and ZIKV transmission. (C) 2021 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. (AU) | |
| Processo FAPESP: | 17/09661-0 - Desenvolvimento de novas estratégias de diagnóstico sorológico para tipagem da infecção prévia pelo vírus Dengue |
| Beneficiário: | Samuel Santos Pereira |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 16/05570-8 - Utilização de proteínas recombinantes do vírus Zika no desenvolvimento de métodos de diagnóstico e aplicações vacinais |
| Beneficiário: | Lennon Ramos Pereira |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 16/20045-7 - Descoberta de antígenos e desenvolvimento de métodos de diagnóstico sorológico e estratégias vacinais contra o Vírus Zika (ZIKV) |
| Beneficiário: | Luis Carlos de Souza Ferreira |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |