| Texto completo | |
| Autor(es): |
Lopes, Laiane A.
[1]
;
Kopp, Willian
[2]
;
Milessi, Thais S.
[3, 4]
;
Padua, Thiago F.
[2]
;
Giordano, Raquel L. C.
[2, 1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Fed Univ Sao Carlos PPEQ UFSCar, Grad Program Chem Engn, Rodovia Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Fed Sao Carlos, Dept Chem Engn, Rodovia Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil
[3] Univ Fed Itajuba, Inst Nat Resources, Av Benedito Pereira dos Santos 1303, BR-37500903 Itajuba, MG - Brazil
[4] Univ Fed Itajuba, Grad Program Energy Engn, Av Benedito Pereira dos Santos 1303, BR-37500903 Itajuba, MG - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Brazilian Journal of Chemical Engineering; NOV 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
New efficient protein purification methods need to be developed to overcome product loss during purification and to lower its final price. Magnetic adsorbents emerge as a potential support which can easily separate target biomolecules from crude biological medium. The hydrophobic adsorption of recombinant lipase from Bacillus thermocatenulatus (BTL-2) in novel silica magnetic microparticles (SMMp) was investigated aiming to directly recover it from cell lysate. The adsorbent surface of SMMp was modified by activation with octyl groups (25%-100%). Adsorptions on silica-octyl (non-magnetic) were also performed to understand the process better. Using centrifuged and dialyzed enzyme, under low ionic strength, the highest enzyme adsorption (92%) and desorption yields (50%) were obtained using SMMp-octyl-75% and SMMp-octyl-25%, respectively, due to simultaneous hydrophobic and ionic interaction between charged silanol groups and the enzyme. Direct enzyme adsorption from cell lysates under high ionic strength conditions led to a high yield of enzyme adsorption with 100% desorption using SMMp-octyl-25%. Therefore, the use of SMMp-octyl allows for a simple and highly efficient BTL-2 recovery by a one-step purification technique that can even be extended to other applications. (AU) | |
| Processo FAPESP: | 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas |
| Beneficiário: | Roberto de Campos Giordano |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Temático |
| Processo FAPESP: | 08/56246-0 - Bioprocess Systems Engineering (BSE) applied to the production of bioethanol from sugarcane bagasse |
| Beneficiário: | Roberto de Campos Giordano |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Temático |