Direct recovery of intracellular lipase from cell lysate by adsorption on silica magnetic microparticles activated with Octyl groups

Author(s)

Lopes, Laiane A. ^[1] ; Kopp, Willian ^[2] ; Milessi, Thais S. ^{[3, 4]} ; Padua, Thiago F. ^[2] ; Giordano, Raquel L. C. ^{[2, 1]}

Affiliation

^[1] Fed Univ Sao Carlos PPEQ UFSCar, Grad Program Chem Engn, Rodovia Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Chem Engn, Rodovia Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[3] Univ Fed Itajuba, Inst Nat Resources, Av Benedito Pereira dos Santos 1303, BR-37500903 Itajuba, MG - Brazil

^[4] Univ Fed Itajuba, Grad Program Energy Engn, Av Benedito Pereira dos Santos 1303, BR-37500903 Itajuba, MG - Brazil

• Document type: Journal article
• Source: Brazilian Journal of Chemical Engineering; NOV 2021.
• Web of Science Citations: 0

Abstract

New efficient protein purification methods need to be developed to overcome product loss during purification and to lower its final price. Magnetic adsorbents emerge as a potential support which can easily separate target biomolecules from crude biological medium. The hydrophobic adsorption of recombinant lipase from Bacillus thermocatenulatus (BTL-2) in novel silica magnetic microparticles (SMMp) was investigated aiming to directly recover it from cell lysate. The adsorbent surface of SMMp was modified by activation with octyl groups (25%-100%). Adsorptions on silica-octyl (non-magnetic) were also performed to understand the process better. Using centrifuged and dialyzed enzyme, under low ionic strength, the highest enzyme adsorption (92%) and desorption yields (50%) were obtained using SMMp-octyl-75% and SMMp-octyl-25%, respectively, due to simultaneous hydrophobic and ionic interaction between charged silanol groups and the enzyme. Direct enzyme adsorption from cell lysates under high ionic strength conditions led to a high yield of enzyme adsorption with 100% desorption using SMMp-octyl-25%. Therefore, the use of SMMp-octyl allows for a simple and highly efficient BTL-2 recovery by a one-step purification technique that can even be extended to other applications. (AU)

• FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
• Grantee: Roberto de Campos Giordano
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 08/56246-0 - Bioprocess Systems Engineering (BSE) applied to the production of bioethanol from sugarcane bagasse
• Grantee: Roberto de Campos Giordano
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants