TOR signaling-related microRNAs as cardiac hypertrophy modulators in high-volume endurance trainin

Autor(es)

Pelozin, Bruno R. A. ^[1] ; Soci, Ursula P. R. ^[1] ; Gomes, Joao L. P. ^[1] ; Oliveira, Edilamar M. ^[1] ; Fernandes, Tiago ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Phys Educ & Sport, Lab Biochem & Mol Biol Exercise, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Physiology; v. 132, n. 1, p. 126-139, JAN 2022.
• Citações Web of Science: 0

Resumo

Aerobic exercise training (ET) promotes cardiovascular adaptations, including physiological left ventricular hypertrophy (LVH). However, the molecular mechanisms underlying these changes are unclear. The study aimed to elucidate specific microRNAs (miRNAs) and target genes involved with the protein kinase B (Akt)/mammalian target of rapamycin (mTOR) signaling in high-volume ET-induced LVH. Eight-week-old female Wistar rats were assigned to three groups: sedentary control (SC), trained protocol 1 (P1), and trained protocol 2 (P2). P1 consisted of 60 min/day of swimming, 5 times/wk, for 10 wk. P2 consisted of the same protocol as P1 until the 8th week; in the 9th week rats trained 2 times/day, and in the 10th week they trained 3 times/day. Subsequently, structure and molecular parameters were evaluated in the heart. Trained groups demonstrate higher values of peak oxygen uptake ( \_VO2peak), exercise tolerance, and LVH in a volume-dependent manner. The miRNA-26a-5p levels were higher in P1 and P2 compared with the SC group (150 +/- 15%, d = 1.8; 148 +/- 16%, d = 1.7; and 100 +/- 7%, respectively; P < 0.05). In contrast, miRNA-16-5p levels were lower in P1 and P2 compared with the SC group (69 +/- 5%, d = 2.3, P < 0.01; 37 +/- 4%, d = 5.6, P < 0.001; and 100 +/- 6%, respectively). Additionally, miRNA-16-5p knockdown and miRNA-26a-5p overexpression significantly promoted cardiomyocyte hypertrophy in neonatal rat cardiomyocytes. Both miRNAs were selected, with the DIANA Tools bioinformatics website, for acting in the mTOR signaling pathway. The protein expression of AKT, MTOR, ribosomal protein S6 kinase beta-1 (P70S6K), and eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) were greater in P1 and even more pronounced in P2. Nonetheless, glycogen synthase kinase 3 beta (GSK3b) protein expression was lower in trained groups. Together, these molecular changes may contribute to a pronounced physiological LVH observed in high-volume aerobic training. NEW \& NOTEWORTHY Physiological hypertrophic growth of the heart as a compensatory response to exercise training (ET) is coupled with recent progress in dissecting the microRNA (miRNA)-mediated molecular basis of hypertrophy. Aerobic ET seems to reduce miRNA-16-5p and increase miRNA-26a-5p expression in a volume-dependent mode, activating protein kinase B (Akt)/ mammalian target of rapamycin (mTOR) pathways, and likely produces an enhanced left ventricular hypertrophy (LVH) in highvolume endurance training. New insight into these mechanisms can be useful in understanding physiological LVH and how it (AU)

• Processo FAPESP: 15/22814-5 - Câncer e coração: novos paradigmas de diagnóstico e tratamento
• Beneficiário: Carlos Eduardo Negrão
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/17275-8 - Papel do microRNA-205 no déficit angiogênico mediado pela sinalização de FAK na hipertensão arterial: efeito do treinamento físico e perspectiva do potencial terapêutico
• Beneficiário: Tiago Fernandes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado