Effects of clinical and metabolic variables and hormones on the expression of immune protein biomarkers in the endometrium of women with polycystic ovary syndrome and normal-cycling controls

Autor(es)

Giordano, Luiz Augusto ; Giordano, Mario Vicente ; Teixeira Gomes, Regina Celia ; Simoes, Ricardo Dos Santos ; Pinheiro Baracat, Maria Candida ; Giordano, Mario Gaspare ; Ferreira-Filho, Edson Santos ; de Medeiros, Sebastiao Freitas ; Baracat, Edmund Chada ; Soares-Junior, Jose Maria

• Tipo de documento: Artigo Científico
• Fonte: Gynecological Endocrinology; v. 38, n. 6, p. 8-pg., 2022-04-08.

Resumo

Background Women with polycystic ovary syndrome (PCOS) are at an elevated risk of endometrial cancer, which may be associated with the continuous proliferative state caused by the interaction between hormones and metabolic factors. Objective To investigate the impact of hormones and metabolic factors in the proliferation and death of endometrium during the proliferative phase. Methods Cross-sectional study with 11 women with PCOS and eight normal-cycling non-PCOS controls at the Federal University of the State of Rio de Janeiro from February 2011 to June 2019. Clinical, biochemical, and hormonal data were collected to analyze their influence on the expression of biomarkers related to the endometrial tissue breakdown. Hysteroscopy and endometrial biopsies were conducted, and the endometrial samples underwent immunohistochemistry for markers of apoptosis B-cell lymphoma 2 (BCL2), cleaved caspase-3 (CASP3), fas cell surface death receptor (FAS), FAS ligand (FASLG), BCL2 associated X (BAX), marker of proliferation Ki-67 (MKI67), and cell death using terminal deoxynucleotidyl transferase dUTP nick and labeling (TUNEL). Results CASP3 and TUNEL expressions were lower in both stroma and endometrium gland of PCOS women than in controls. MKI67 and homeostasis indexes (BCL2/BAX; FASLG/FAS) in the endometrium of the PCOS group were significantly higher. Body mass index (BMI) values were positively correlated with the expression of MKI67 and MKI67/TUNEL ratio in the endometrial stroma compartment. Fasting insulin levels were positively correlated with the expression of BCL2, and DHEA-S levels were negatively correlated with the expression of CASP3 of women with PCOS. Conclusion BMI, insulin, and DHEA-S influence the endometrial homeostasis breakdown in PCOS in the endometrium stroma. (AU)

• Processo FAPESP: 09/54019-9 - Efeito da metformina na resistencia insulinica e na melatonina em adolescentes com sindrome dos ovarios policisticos
• Beneficiário: José Maria Soares Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/24224-9 - Efeitos extrareprodutivos da melatonina sobre o sistema genital e mamário
• Beneficiário: José Maria Soares Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático