Violacein negatively modulates the colorectal cancer survival and epithelial-mesenchymal transition

Autor(es)

de Souza Oliveira, Patricia F. ; Faria, Alessandra V. S. ; Clerici, Stefano P. ; Akagi, Erica M. ; Carvalho, Hernandes F. ; Justo, Giselle Z. ; Duran, Nelson ; Ferreira-Halder, Carmen, V

• Tipo de documento: Artigo Científico
• Fonte: Journal of Cellular Biochemistry; v. N/A, p. 12-pg., 2022-06-06.

Resumo

Violacein is a secondary metabolite produced by several microorganisms including Chromobacterium violaceum, and it is already used in food and cosmetics. However, due to its potent anticancer and low side effects, its molecular action needs to be deeply scrutinized. Therefore, the main objective of this study was to evaluate the violacein's ability to interfere with three cancer hallmarks: growth factors receptor-dependent signaling, proliferation, and epithelial-mesenchymal transition (EMT). Violacein has been associated with the induction of apoptosis in colorectal cancer (CRC) cells. Here, we demonstrate that this molecule is also active in CRC spheroids and inhibits cell migration. Violacein treatment reduced the amount of EGFR and AXL receptors in the HT29 cell line. Accordingly, the inhibition of the AKT, ERK, and PKC delta kinases, which are downstream mediators of the signaling pathways triggered by EGFR and AXL, is detected. Another interesting finding was that even when the cells were stimulated with transforming growth factor-beta, the EMT marker (N-cadherin) decreased. Therefore, this study provides further evidence that reinforces the potential of violacein as an antitumor agent, once this biomolecule can "switch off" properties associated with cancer plasticity. (AU)

• Processo FAPESP: 17/16625-0 - Importância da proteína tirosina fosfatase de baixo peso molecular na reprogramação do metabolismo tumoral
• Beneficiário: Erica Mie Akagi da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/03593-6 - Educação plaquetária e metástase: relevância da LMWPTP e vesículas extracelulares no Câncer Colorretal
• Beneficiário: Stefano Piatto Clerici
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/50938-8 - INCT 2014: em Fotônica Aplicada à Biologia Celular INFABiC
• Beneficiário: Hernandes Faustino de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 20/04409-4 - Esferoides e organoides de câncer gástrico: rastreamento de proteínas quinases e da arquitetura da matriz extracelular visando impacto terapêutico
• Beneficiário: Carmen Veríssima Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/08119-8 - Primeira etapa da disseminação hematogênica de células de câncer de cólon retal: influência da LMWPTP e do 3-bromopiruvato
• Beneficiário: Alessandra Valéria de Sousa Faria
• Modalidade de apoio: Bolsas no Brasil - Doutorado