Lack of serological and molecular evidences of Zika virus circulation in non-human primates in three states from Brazil

Autor(es):Mostrar menos -

Amanda Haisi ^[1] ; Stacy Wu ^[2] ; Nathalia Zini ^[3] ; Maria Luana Cristiny Rodrigues da Silva ^[4] ; Camila Dantas Malossi ^[5] ; Zalmir Silvino Cubas ^[6] ; Patrícia Hoerner Cubas ^[7] ; Rodrigo Hidalgo Friciello Teixeira ; Mônica Shinneider de Sousa ^[9] ; Ricardo Barbosa Lucena ^[10] ; Walfrido Kühl Svoboda ^[11] ; Silvia Cristina Osaki ^[12] ; Mauricio Lacerda Nogueira ; Leila Sabrina Ullmann ; João Pessoa Araújo Junior ^[15]

Afiliação do(s) autor(es):Mostrar menos -

^[1] Universidade Estadual Paulista Júlio de Mesquita Filho. Instituto de Biotecnologia - Brasil

^[2] Universidade Federal do Paraná. Departamento de Ciências Veterinárias - Brasil

^[3] Escola de Medicina de São José do Rio Preto. Laboratório de Pesquisas em Virologia - Brasil

^[4] Universidade Federal de Campina Grande - Brasil

^[5] Universidade Estadual Paulista Júlio de Mesquita Filho. Instituto de Biotecnologia - Brasil

^[6] Refúgio Biológico Bela Vista. Itaipu Binacional - Brasil

^[7] Zoológico Bosque do Guarani - Brasil

^[9] Universidade Federal de Campina Grande - Brasil

^[10] Universidade Federal da Paraíba. Departamento de Ciências Veterinárias - Brasil

^[11] Universidade Federal da Integração Latino-Americana - Brasil

^[12] Universidade Federal do Paraná. Departamento de Ciências Veterinárias - Brasil

^[15] Universidade Estadual Paulista Júlio de Mesquita Filho. Instituto de Biotecnologia - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Memórias do Instituto Oswaldo Cruz; v. 117, 2022-09-05.

Resumo

BACKGROUND Zika virus (ZIKV) was discovered in 1947 with the virus isolation from Rhesus monkey (Macaca mulatta) in Uganda forest, Africa. Old World Primates are involved in a sylvatic cycle of maintenance of this arbovirus, however a limited knowledge about the role of New World primates in ZIKV transmission cycles has been established. OBJECTIVE This work aimed to investigate the presence of enzootic circulation of ZIKV in New World Primates from three Brazilian states: São Paulo, Paraíba, and Paraná. METHODS We analyzed 100 non-human primate samples collected in 2018 and 2020 from free-ranging and captive environments from São Paulo (six municipalities belonging to Sorocaba region), Paraíba (João Pessoa municipality), and Paraná (Foz do Iguaçu municipality) using reverse transcriptase quantitative polymerase reaction (RT-qPCR) assays, indirect enzyme-linked immunosorbent assay (ELISA), and plaque reduction neutralization test (PRNT). FINDINGS All samples (n = 141) tested negative for the presence of ZIKV genome from tissue and blood samples. In addition, all sera (n = 58) from Foz do Iguaçu’ non-human primates (NHPs) were negative in serological assays. MAIN CONCLUSION No evidence of ZIKV circulation (molecular and serological) was found in neotropical primates. In addition, the absence of antibodies against ZIKV suggests the absence of previous viral exposure of NHPs from Foz do Iguaçu-PR. (AU)

• Processo FAPESP: 19/05288-9 - Detecção molecular e análise filogenética do zika vírus (ZIKV) em primatas não-humanos (PNH) do Estado de São Paulo e Paraná, Brasil
• Beneficiário: Amanda Haisi
• Modalidade de apoio: Bolsas no Brasil - Mestrado