Synergic recombinant enzyme association to optimize xylo-oligosaccharides production from agricultural waste

Autor(es)

Martins, Manoela ; Avila, Patricia Felix ; Paim de Andrade, Cristiane Conte ; Goldbeck, Rosana

• Tipo de documento: Artigo Científico
• Fonte: BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY; v. 28, p. 8-pg., 2020-09-01.

Resumo

Xylo-oligosaccharides (XOS) are one of the classes of prebiotics obtained from lignocellulosic biomass, by hydrolysis of hemicellulose, abundant in agroindustrial residues. Several endoxylanases were studied in order to provide better conversion of the biomass to XOS. The resulting product presents multi-benefits, favoring the intestinal microbiota and maintaining the viability of probiotics in food. XOS production from sugarcane straw by action of thermostable recombinant endoxylanase of Cryptococcus fiavescens, expressed in Pichia pastoris GS115 using vector pGAPZ alpha A, in synergic association with alpha-L-arabinofuranosidase (GH 51) (Megazyme (R)) was optimized using a central composite rotatable design (CCRD), enabling 72.56% of xylan conversion. The main products xylobiose and xylotriose are short chain oligosaccharides preferred by probiotics. Hydrolysis of xylan from sugarcane straw using endoxylanase and alpha-L-arabinofuranosidase has great potential for XOS production with high prebiotic activity, representing a possible sustainable way to reduce enzymatic and raw material costs. (AU)

• Processo FAPESP: 15/20630-4 - Desenvolvimento integrado de biorrefinaria e planta de bioetanol de cana-de-açúcar com emissão zero de CO2: rotas para converter recursos renováveis em bioprodutos e bioeletricidade
• Beneficiário: Rubens Maciel Filho
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/14223-5 - Obtenção de microcápsulas simbióticas a partir de xilo-oligossacarídeos oriundos de palha de cana-de-açúcar
• Beneficiário: Manoela Martins
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 15/50612-8 - An integrated approach to explore a novel paradigm for biofuel production from lignocellulosic feedstocks
• Beneficiário: Telma Teixeira Franco
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/08542-3 - Biotecnologia enzimática para produção de xilo-oligossacarídeos a partir de resíduos agroindustriais lignocelulósicos visando a produção de simbióticos
• Beneficiário: Rosana Goldbeck Coelho
• Modalidade de apoio: Auxílio à Pesquisa - Regular