Role of antigen-presenting cells in non-ulcerated skin lesions caused by Leishmania (Leishmania) infantum chagasi

Autor(es)

Pacheco, Carmen M. Sandoval M. ; Flores, Gabriela V. Araujo V. ; Ferreira, Aurea F. F. ; da Matta, Vania L. R. ; Gomes, Claudia M. de Castro M. ; Sosa-Ochoa, Wilfredo H. H. ; Zuniga, Concepcion ; Silveira, Fernando T. T. ; Corbett, Carlos E. P. ; Laurenti, Marcia D.

• Tipo de documento: Artigo Científico
• Fonte: PARASITE IMMUNOLOGY; v. N/A, p. 11-pg., 2023-01-31.

Resumo

In Central America, infection by Leishmania (Leishmania) infantum chagasi causes visceral leishmaniasis and non-ulcerated cutaneous leishmaniasis (NUCL). This work aimed to evaluate the participation of subpopulations of antigen-presenting cells in skin lesions of patients affected by NUCL through double-staining immunohistochemistry using cellular and intracellular markers. Twenty-three skin biopsies from patients affected by NUCL were used. Histological sections stained by HE were used for histopathological study. Immunohistochemical studies were performed using primary antibodies against Langerhans cells, dermal dendritic cells, T lymphocytes, and the cytokines IL-12, IFN-gamma, TNF-alpha, iNOS, and IL-10. The histopathological lesions were characterized by an inflammatory infiltrate, predominantly lymphohistiocytic, of variable intensity, with a diffuse arrangement associated with epithelioid granulomas and discreet parasitism. Double-staining immunohistochemistry showed higher participation of dendritic cells producing the proinflammatory cytokine IL-12 in relation to the other evaluated cytokines. Activation of the cellular immune response was marked by a higher density of CD8 Tc1-lymphocytes followed by CD4 Th1-lymphocytes producing mainly IFN-gamma. The data obtained in the present study suggest that antigen-presenting cells play an important role in the in situ immune response through the production of proinflammatory cytokines, directing the cellular immune response preferentially to the Th1 and Tc1 types in NUCL caused by L. (L.) infantum chagasi. (AU)

• Processo FAPESP: 18/04698-6 - Leishmaniose cutânea não ulcerada ou atípica causada pela Leishmania (L.) infantum chagasi no município de Amapala, Valle, Honduras: caracterização imuno-histopatológica das lesões de pele com ênfase na participação das células apresentadoras de antígenos
• Beneficiário: Carmen Maria Sandoval Pacheco
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/24834-9 - Avaliação da evolução da infecção experimental em hamster por parasitos isolados de lesão cutânea atípica e visceral humana causada por Leishmania (L.) infantum chagasi no município de Amapala, Valle, Honduras
• Beneficiário: Gabriela Venicia Araujo Flores
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/50315-0 - Leishmanioses na América Latina: uma perspectiva avançada sobre fatores imunopatogenéticos da infecção cutânea e visceral, imunomoduladores da saliva de vetores flebotomíneos e exo-antígenos imunogênicos de Leishmania (L.) infantum chagasi candidatos à vacina
• Beneficiário: Marcia Dalastra Laurenti
• Modalidade de apoio: Auxílio à Pesquisa - Temático