| Texto completo | |
| Autor(es): Mostrar menos - |
Capetini, Vinicius Cooper
;
Quintanilha, Bruna J.
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de Oliveira, Dalila Cunha
;
Nishioka, Alessandra Harumi
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de Matos, Luciene Assaf
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Ferreira, Ludmila Rodrigues Pinto
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Ferreira, Frederico Moraes
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Sampaio, Geni Rodrigues
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Hassimotto, Neuza Mariko Aymoto
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Lajolo, Franco Maria
;
Fock, Ricardo Ambrosio
;
Rogero, Marcelo Macedo
Número total de Autores: 12
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| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF NUTRITIONAL BIOCHEMISTRY; v. 112, p. 14-pg., 2022-12-17. |
| Resumo | |
Blood orange consumption presents potential health benefits and may modulate epigenetic mechanisms such as microRNAs (miRNAs) expression. MiR-NAs are non-coding RNAs responsible for post-transcriptional gene regulation, and these molecules can also be used as biomarkers in body fluids. This study was designed to investigate the effect of chronic blood orange juice (BOJ) intake on the inflammatory response and miRNA expression profile in plasma and blood cells in overweight women. The study cohort was comprised of twenty women aged 18-40 years old, diagnosed as overweight, who consumed 500 mL/d of BOJ for four weeks. Clinical data were collected at baseline and after 4 weeks of juice consumption, e.g., anthropometric and hemodynamic parameters, food intake, blood cell count, and metabolic and inflammatory biomarkers. BOJ samples were analyzed and characterized. Additionally, plasma and blood cells were also collected for miRNA expression profiling and evaluation of the expression of genes and proteins in the MAPK and NF Kappa B signaling pathways. BOJ intake increased the expression of miR-144-3p in plasma and the expression of miR-424-5p, miR-144-3p, and miR-130b-3p in peripheral blood mononuclear cells (PBMC). Conversely, the beverage intake decreased the expression of let-7f-5p and miR-126-3p in PBMC. Computational analy-ses identified different targets of the dysregulated miRNA on inflammatory pathways. Furthermore, BOJ intake increased vitamin C consumption and the pJNK/JNK ratio and decreased the expression of IL6 mRNA and NF Kappa B protein. These results demonstrate that BOJ regulates the expression of genes involved in the inflammatory process and decreases NF k B-protein expression in PBMC.(c) 2022 Elsevier Inc. All rights reserved. (AU) | |
| Processo FAPESP: | 18/25046-7 - Efeito crônico da ingestão de suco de laranja sanguínea sobre o perfil de expressão de microRNA e resposta inflamatória em mulheres com sobrepeso |
| Beneficiário: | Vinícius Cooper Capetini |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 13/07914-8 - FoRC - Centro de Pesquisa em Alimentos |
| Beneficiário: | Bernadette Dora Gombossy de Melo Franco |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |