A phytotherapic blend immunity-6TM inhibits myeloid leukemic cells 2 activation involving purinergic signaling

Autor(es)

Bella, Yanesko Fernandes ; Oliveira, Carlos Rocha ; Mateus-Silva, Jose Roberto ; Brandao-Rangel, Maysa Alves Rodrigues ; Silva-Reis, Anamei ; Santos, Juliana de Melo Batista ; Albertini, Regiane ; Lopes-Martins, Rodrigo Alvaro Brandao ; de Oliveira, Luis Vicente Franco ; Vieira, Rodolfo P.

• Tipo de documento: Artigo Científico
• Fonte: BIOMEDICINE & PHARMACOTHERAPY; v. 159, p. 6-pg., 2023-01-16.

Resumo

Leukemia is among the most common types of hematological cancers and the use of herbal medicines to prevent and treat leukemia are under quick development. Among several molecular pathways involved in leukemia pathogenesis and exacerbations, purinergic signaling is revealed as a key component. In the present study, the effects of two doses (5 ug/mL and 10 ug/mL) of Immunity-6TM, a phytocomplex composed by beta-glucan, green tea (Camelia sinensis), chamomile (Matricaria chamomilla), and ascorbic acid (vitamin C) was tested in vitro, using chronic myelogenous leukemia cell line (K-562; 5 x104/mL/well), which were challenged with lipo-polysaccharide (LPS; 1 ug/mL) for 24 h. The results demonstrated that both doses of Immunity-6TM inhibited ATP release (p < 0.001) and P2x7 receptor at mRNA levels expression (p < 0.001). Purinergic inhibition by Im-munity-6TM was followed by reduced release of proinflammatory cytokines IL-1beta (p < 0.001) and IL-6 (p < 0.001), while only 5 ug/mL of Immunity-6TM reduced the release of TNF-alpha (p < 0.001). Beyond to inhibit the release of pro-inflammatory cytokines, both doses of Immunity-6TM induced the release of anti-inflammatory cytokine IL-10 (p < 0.001), while only the higher dose (10 ug/mL) of Immunity-6TM induced the release of anti-inflammatory IL-1ra (p < 0.05) and klotho (p < 0.001). Thus, Immunity-6TM may be a promising adjuvant in the treatment of leukemia and further clinical trials are guaranteed. (AU)

• Processo FAPESP: 19/05739-0 - Papel das cininas nos efeitos anti-inflamatórios do treinamento aeróbio em modelo experimental de Asma
• Beneficiário: Maysa Alves Rodrigues Brandão Rangel
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/15165-2 - Papel da sinalização purinérgica e da sinalização SOCS-JAK-STAT nos efeitos antiinflamatórios do treinamento aeróbio em modelos experimentais de asma e em indivíduos asmáticos
• Beneficiário: Rodolfo de Paula Vieira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 19/11244-4 - Efeitos do treinamento físico combinado sobre a mecânica e função pulmonar e sobre a resposta imune pulmonar e sistêmica de mulheres obesas
• Beneficiário: Anamei Silva Reis
• Modalidade de apoio: Bolsas no Brasil - Mestrado