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Discriminating aspects of global metabolism of neonatal cardiomyocytes from wild type and KO-CSRP3 rats using proton magnetic resonance spectroscopy of culture media samples

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Autor(es):
Bloise, Antonio Carlos ; dos Santos, Jennifer Adriane ; de Brito, Isis Vasconcelos ; Bassaneze, Vinicius ; Gomes, Ligia Ferreira ; Alencar, Adriano Mesquita
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL; v. 56, n. 8, p. 10-pg., 2020-09-10.
Resumo

Knockout of multifunction gene cysteine- and glycine-rich protein 3 (CSRP3) in cardiomyocytes (CMs) of mice leads to heart dilation, severely affecting its functions. In humans, CSRP3 mutations are associated with hypertrophic (HCM) and dilated cardiomyopathy (DCM). The absence of the CSRP3 expression produces unknown effects on in vitro neonatal CMs' metabolism. The metabolome changes in culture media conditioned by CSRP3 knockout (KO-CSRP3), and wild type (WT) neonatal cardiomyocytes were investigated under untreated or after metabolic challenging conditions produced by isoproterenol (ISO) stimulation, by in vitro high-resolution proton magnetic resonance spectroscopy (H-1-MRS)-based metabolomics. Metabolic differences between neonatal KO-CSRP3 and WT rats' CMs were identified. After 72 h of culture, ISO administration was associated with increased CMs' energy requirements and increased levels of threonine, alanine, and 3-hydroxybutyrate in both neonatal KO-CSRP3 and WT CMs conditioned media. When compared with KO-CSRP3, culture media derived from WT cells presented higher lactate concentrations either under basal or ISO-stimulated conditions. The higher activity of ketogenic biochemical pathways met the elevated energy requirements of the contractile cells. Both cells are considered phenotypically indistinguishable in the neonatal period of animal lives, but the observed metabolic stress responses of KO-CSRP3 and WT CMs to ISO were different. KO-CSRP3 CMs produced less lactate than WT CMs in both basal and stimulated conditions. Mainly, ISO-stimulated conditions produced evidence for lactate overload within KO-CSRP3 CMs, while WT CMs succeeded to manage the metabolic stress. Thus,H-1-MRS-based metabolomics was suitable to identify early inefficient energetic metabolism in neonatal KO-CSRP3 CMs. These results may reflect an apparent lower lactate transport and consumption, in association with protein catabolism. (AU)

Processo FAPESP: 14/22102-2 - Caracterização da dinâmica de cardiomiócitos por microscopia de força tração (TFM) e Speckle
Beneficiário:Adriano Mesquita Alencar
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/21646-9 - Caracterização das Atividades de Células Vivas Através de Técnicas Interferométricas: Speckle e Microscopia Holográfica Digital
Beneficiário:Isis Vasconcelos de Brito
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 18/20910-5 - Avaliação das Propriedades Fenotípicas de Células Cardíacas Cultivadas em Micropadrões
Beneficiário:Jennifer Adriane dos Santos
Modalidade de apoio: Bolsas no Brasil - Doutorado
Processo FAPESP: 11/19678-1 - Identificação e isolamento de células progenitoras cardíacas usando aptâmeros
Beneficiário:Vinícius Bassaneze
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 13/17368-0 - Genômica cardiovascular: mechanismos & novas terapias - CVGen mech2ther
Beneficiário:José Eduardo Krieger
Modalidade de apoio: Auxílio à Pesquisa - Temático