Antibody cross-reactivity and evidence of susceptibility to emerging Flaviviruses in the dengue-endemic Brazilian Amazon

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Salgado, Barbara Batista ; Maues, Fabio Carmona de Jesus ; Jordao, Maele ; Pereira, Renato Lemos ; Toledo-Teixeira, Daniel A. ; Parise, Pierina L. ; Granja, Fabiana ; Souza, Higo Fernando Santos ; Yamamoto, Marcio Massao ; Chiang, Jannifer Oliveira ; Martins, Livia Caricio ; Boscardin, Silvia Beatriz ; Lalwani, Jaila Dias Borges ; Vasconcelos, Pedro Fernando C. ; Proenca-Modena, Jose Luiz ; Lalwani, Pritesh

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES; v. 129, p. 10-pg., 2023-02-21.

Resumo

Objectives: Several Flaviviruses can co-circulate. Pre-existing immunity to one virus can modulate the response to a heterologous virus; however, the serological cross-reaction between these emerging viruses in dengue virus (DENV)-endemic regions are poorly understood. Methods: A cross-sectional study was performed among the residents of Manaus city in the state of Amazonas, Brazil. The serological response was assessed by hemagglutination inhibition assay (HIA), enzymelinked immunosorbent assay, and neutralization assay. Results: A total of 74.52% of the participants were immunoglobulin G-positive (310/416), as estimated by lateral flow tests. Overall, 93.7% of the participants were seropositive (419/447) for at least one DENV serotype, and the DENV seropositivity ranged between 84.8% and 91.0%, as determined by HIA. About 93% had antiyellow fever virus 17D-reactive antibodies, whereas 80.5% reacted to wild-type yellow fever virus. Zika virus (ZIKV) had the lowest seropositivity percentage (52.6%) compared with other Flaviviruses. Individuals who were DENV-positive with high antibody titers by HIA or envelope protein domain III enzymelinked immunosorbent assay reacted strongly with ZIKV, whereas individuals with low anti-DENV antibody titers reacted poorly toward ZIKV. Live virus neutralization assay with ZIKV confirmed that dengue serogroup and ZIKV-spondweni serogroup are far apart; hence, individuals who are DENV-positive do not cross-neutralize ZIKV efficiently. Conclusion: Taken together, we observed a high prevalence of DENV in the Manaus-Amazon region and a varying degree of cross-reactivity against emerging and endemic Flaviviruses. Epidemiological and exposure conditions in Manaus make its population susceptible to emerging and endemic arboviruses. (c) 2023 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. ( http://creativecommons.org/licenses/by-nc-nd/4.0/ ) (AU)

• Processo FAPESP: 16/00194-8 - Patogênese e neurovirulência de vírus emergentes no Brasil
• Beneficiário: José Luiz Proença Módena
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 20/04558-0 - Caracterização de fatores de risco intrínsecos e o desenvolvimento de novas alternativas de diagnóstico e tratamento para COVID-19
• Beneficiário: José Luiz Proença Módena
• Modalidade de apoio: Auxílio à Pesquisa - Regular