Biotransformation of white and black mustard grains through germination and enzymatic hydrolysis revealed important metabolites for antioxidant properties and cytotoxic activity against Caco-2 cells

Autor(es)

Rasera, Gabriela Boscariol ; de Vilhena Araujo, Eder ; Pereira, Alana Kelyene ; Liszbinski, Raquel Bester ; Pacheco, Guilherme ; Fill, Taicia Pacheco ; de Jesus, Marcelo Bispo ; de Castro, Ruann Janser Soares

• Tipo de documento: Artigo Científico
• Fonte: Food Research International; v. 169, p. 12-pg., 2023-05-11.

Resumo

Germination and enzymatic hydrolysis are biological processes with well-recognized positive effects on phenolic composition and antioxidant potential. This study aimed to apply those processes to white (Sinapsis alba) and black (Brassica nigra) mustard grains and to analyze the influences on the total phenolic content (TPC); phenolic and peptide profile determined by ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS); antioxidant potential (DPPH, ABTS, and FRAP assays); and cytotoxicity against Caco-2, a human colorectal adenocarcinoma cell line. Enzyme combinations for hydrolysis were different for each mustard grain, but for both species, enzymatic hydrolysis and germination showed a positive effect on antioxidant properties. From UPLC-HRMS analysis and molecular network studies, 14 peptides and 17 phenolic compounds were identified as metabolites released from mustard after processes application, which were strongly correlated with increased antioxidant activity. In addition, enzymatic hydrolysis applied in germinated mustard grains for both mustards increased the cytotoxic activity against Caco-2 human colorectal adenocarcinoma cell line. (AU)

• Processo FAPESP: 19/06023-9 - Investigação dos mecanismos moleculares induzidos por vetores nanoestruturados para transfecção de genes em culturas tumorais esferóides
• Beneficiário: Raquel Bester Liszbinski
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 21/12290-0 - Como a germinação e a hidrólise enzimática podem afetar as propriedades biológicas e a bioacessibilidade de compostos bioativos em grãos de mostarda?
• Beneficiário: Ruann Janser Soares de Castro
• Modalidade de apoio: Auxílio à Pesquisa - Regular