| Texto completo | |
| Autor(es): |
Bezerra, Rafael S.
;
Bitencourt, Hellen T.
;
Covas, Dimas T.
;
Kashima, Simone
;
Slavov, Svetoslav N.
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES; v. 98, p. 3-pg., 2020-09-01. |
| Resumo | |
Objectives: To metagenomically analyse blood units originating from the Brazilian Amazon and positive for parenterally transmitted infections (human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV), human T-lymphotropic virus (HTLV), Chagas disease or syphilis). Methods: Twenty plasma samples (35% HBV-positive, 10% HIV-positive, 10% HCV-positive, 20% positive for syphilis, 20% for Chagas disease, and 5% for HTLV) assembled in pools were analysed by metagenomic next-generation sequencing. The obtained raw sequencing data were submitted to a bioinformatic pipeline set up for identification of emerging viruses. The viral reads of interest were phylogenetically analysed and confirmed by PCR in the individual samples. Results: The metagenomic analysis identified contigs belonging to the emerging human Gemykibivirus-2 (HuGkV-2) in two pools. The HuGkV-1 phylogeny demonstrated that the Amazonian isolate formed a separate cluster with other HuGkV-2 strains obtained from human hosts. The PCR confirmation detected HuGkV-1 DNA in three individual samples (15%). Conclusions: HuGkV-2 is an emerging virus with unknown clinical impact. The detection of HuGkV-2 DNA in blood donations positive for parenterally transmitted infections showed that HuGkV-2 can be considered as an opportunistic viral agent with a hypothetic parenteral transmission route. (C) 2020 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. (AU) | |
| Processo FAPESP: | 19/08528-0 - Aplicação de métodos de bioinformática para identificação de infecções virais com impacto em hemoterapia |
| Beneficiário: | Rafael dos Santos Bezerra |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 18/15826-5 - Avaliação do impacto de vírus emergentes e re-emergentes em hemoterapia e transplante de células-tronco por meio de técnicas moleculares avançadas |
| Beneficiário: | Svetoslav Nanev Slavov |
| Modalidade de apoio: | Bolsas no Brasil - Jovens Pesquisadores |
| Processo FAPESP: | 19/07861-8 - Desenvolvimento de um pipeline de bioinformática para identificação de doenças infecciosas emergentes em pacientes em regime de transfusão crônica |
| Beneficiário: | Ian Nunes Valença |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 17/23205-8 - Avaliação do impacto de vírus emergentes e re-emergentes em hemoterapia e transplante de células-tronco por meio de técnicas moleculares avançadas |
| Beneficiário: | Svetoslav Nanev Slavov |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |