Nafamostat mesilate, a potent tryptase inhibitor, modulates periodontitis in rats

Autor(es)

Holzhausen, Marinella ^[1] ; Balejo, Rodrigo D. P. ^[2] ; Lara, Guilherme M. ^[2] ; Cortelli, Sheila C. ^[2] ; Saad, Wilson A. ^[3] ; Cortelli, Jose R. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent, Div Periodont, Dept Stomatol, Sao Paulo - Brazil

^[2] Univ Taubate, Dept Periodontol, Fac Dent, Taubate, SP - Brazil

^[3] Univ Taubate, Dept Physiol & Pharmacol, Inst Biosci, Taubate, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL ORAL INVESTIGATIONS; v. 15, n. 6, p. 967-973, DEC 2011.
• Citações Web of Science: 7

Resumo

Previous reports have demonstrated increased tryptase-like proteolytic activity in the crevicular fluid of patients with periodontal disease. In the present study, we have investigated the effect of tryptase inhibition with nafamostat mesilate (NM, 6-amino-2-naphtlyl p-guanidinobenzoate dimethansulfonate) on the development of experimental periodontitis in rats. Eighty (80) male Wistar rats were randomly separated into four groups: Control group, NM group (daily 0.1 mg/kg body weight of NM, i.p.), Ligature group (ligature placed at lower right first molars), and NM+Ligature group. The amount of alveolar bone loss (ABL) around the mesial root surface of the first mandibulary molar, as well as the myeloperoxidase (MPO) activity, and total proteolytic activity {[}N-benzoyl-l-arginine-p-nitroanilide (BApNA) substrate] were determined at 7 and 14 days. NM led to significantly (p < 0.05) decreased ABL in animals subjected to ligature-induced periodontitis. Tryptase inhibition prevented the onset of significant ABL at 7 days of experiment (0.44 +/- 0.16 and 0.60 +/- 0.22, p > 0.05, NM+Ligature and Control, respectively) and significantly decreased the ABL at 14 days (0.97 +/- 0.17 versus 1.82 +/- 0.26, p < 0.001, NM+Ligature versus Ligature, respectively). In addition, NM significantly decreased MPO and total proteolytic activity at 14 days (p < 0.05). These data provided evidence that tryptase inhibition with NM attenuates gingival granulocyte infiltration and ABL in an experimental model of periodontitis in rats. (AU)

• Processo FAPESP: 07/50665-8 - Associação do receptor tipo 2 ativado por protease (PAR2) com a doença periodontal em humanos
• Beneficiário: Marinella Holzhausen Caldeira
• Modalidade de apoio: Auxílio à Pesquisa - Regular