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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Nafamostat mesilate, a potent tryptase inhibitor, modulates periodontitis in rats

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Author(s):
Holzhausen, Marinella [1] ; Balejo, Rodrigo D. P. [2] ; Lara, Guilherme M. [2] ; Cortelli, Sheila C. [2] ; Saad, Wilson A. [3] ; Cortelli, Jose R. [2]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Sch Dent, Div Periodont, Dept Stomatol, Sao Paulo - Brazil
[2] Univ Taubate, Dept Periodontol, Fac Dent, Taubate, SP - Brazil
[3] Univ Taubate, Dept Physiol & Pharmacol, Inst Biosci, Taubate, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: CLINICAL ORAL INVESTIGATIONS; v. 15, n. 6, p. 967-973, DEC 2011.
Web of Science Citations: 7
Abstract

Previous reports have demonstrated increased tryptase-like proteolytic activity in the crevicular fluid of patients with periodontal disease. In the present study, we have investigated the effect of tryptase inhibition with nafamostat mesilate (NM, 6-amino-2-naphtlyl p-guanidinobenzoate dimethansulfonate) on the development of experimental periodontitis in rats. Eighty (80) male Wistar rats were randomly separated into four groups: Control group, NM group (daily 0.1 mg/kg body weight of NM, i.p.), Ligature group (ligature placed at lower right first molars), and NM+Ligature group. The amount of alveolar bone loss (ABL) around the mesial root surface of the first mandibulary molar, as well as the myeloperoxidase (MPO) activity, and total proteolytic activity {[}N-benzoyl-l-arginine-p-nitroanilide (BApNA) substrate] were determined at 7 and 14 days. NM led to significantly (p < 0.05) decreased ABL in animals subjected to ligature-induced periodontitis. Tryptase inhibition prevented the onset of significant ABL at 7 days of experiment (0.44 +/- 0.16 and 0.60 +/- 0.22, p > 0.05, NM+Ligature and Control, respectively) and significantly decreased the ABL at 14 days (0.97 +/- 0.17 versus 1.82 +/- 0.26, p < 0.001, NM+Ligature versus Ligature, respectively). In addition, NM significantly decreased MPO and total proteolytic activity at 14 days (p < 0.05). These data provided evidence that tryptase inhibition with NM attenuates gingival granulocyte infiltration and ABL in an experimental model of periodontitis in rats. (AU)