Bothrops jararaca venom proteome rearrangement upon neonate to adult transition

Autor(es)

Zelanis, Andre ^{[1, 2]} ; Tashima, Alexandre K. ^{[3, 2]} ; Pinto, Antonio F. M. ^[4] ; Leme, Adriana F. Paes ^[5] ; Stuginski, Daniel R. ^[6] ; Furtado, Maria F. ^[6] ; Sherman, Nicholas E. ^[7] ; Ho, Paulo L. ^{[8, 1]} ; Fox, Jay W. ^[7] ; Serrano, Solange M. T. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Bioquim, Inst Quim, BR-05508 Sao Paulo - Brazil

^[2] Inst Butantan, Lab Especial Toxinol Aplicada CAT CEPID, BR-05503900 Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Ciencias Exatas & Terra, Sao Paulo - Brazil

^[4] Ctr Biotecnol UFRGS, Lab Bioquim Farmacol, Porto Alegre, RS - Brazil

^[5] LNBio, Lab Nacl Biociencias, Campinas - Brazil

^[6] Inst Butantan, Lab Herpetol, BR-05503900 Sao Paulo - Brazil

^[7] Univ Virginia, Dept Microbiol, Charlottesville, VA 22908 - USA

^[8] Inst Butantan, Ctr Biotecnol, BR-05503900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PROTEOMICS; v. 11, n. 21, p. 4218-4228, NOV 2011.
• Citações Web of Science: 49

Resumo

The pharmacological activities displayed by Bothrops jararaca venom undergo a significant ontogenetic shift. Similarly, the diet of this species changes from ectothermic prey in early life to endothermic prey in adulthood. In this study we used large and representative newborn and adult venom samples consisting of pools from 694 and 110 specimens, respectively, and demonstrate a significant ontogenetic shift in the venom proteome complexity of B. jararaca. 2-DE coupled to MS protein identification showed a clear rearrangement of the toxin arsenal both in terms of the total proteome, as of the glycoproteome. N-glycosylation seems to play a key role in venom protein variability between newborn and adult specimens. Upon the snake development, the subproteome of metalloproteinases undergoes a shift from a P-III-rich to a P-I-rich profile while the serine proteinase profile does not vary significantly. We also used isobaric tag labeling (iTRAQ) of venom tryptic peptides for the first time to examine the quantitative changes in the venom toxins of B. jararaca upon neonate to adult transition. The iTRAQ analysis showed changes in various toxin classes, especially the proteinases. Our study expands the in-depth understanding of venom complexity variation particularly with regard to toxin families that have been associated with envenomation pathogenesis. (AU)

• Processo FAPESP: 04/14846-0 - Rede de proteoma do estado de São Paulo
• Beneficiário: Fabio Cesar Gozzo
• Modalidade de apoio: Auxílio à Pesquisa - Programa GENOMA
• Processo FAPESP: 07/54626-7 - Proteômica e transcriptômica aplicadas ao estudo das variações na composição do veneno de serpentes do gênero Bothrops relacionadas à idade e ao sexo
• Beneficiário: Solange Maria de Toledo Serrano
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 98/14307-9 - Center for Applied Toxinology
• Beneficiário: Hugo Aguirre Armelin
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs