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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Porphyromonas Gingivalis is Associated With Protease-Activated Receptor-2 Upregulation in Chronic Periodontitis

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Autor(es):
Fagundes, Jose Americo G. [1] ; Monoo, Leticia D. [1] ; Euzebio Alves, Vanessa Tubero [2] ; Pannuti, Claudio Mendes [2] ; Cortelli, Sheila Cavalca [1] ; Cortelli, Jose Roberto [1] ; Holzhausen, Marinella [2]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Taubate, Dept Periodontol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Div Periodont, Dept Stomatol, Sch Dent, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Journal of Periodontology; v. 82, n. 11, p. 1596-1601, NOV 2011.
Citações Web of Science: 20
Resumo

Background: We previously reported a higher expression of protease-activated receptor-2 (PAR(2)) together with higher interleukin (IL)-1 alpha, IL-6, IL-8, and tumor necrosis factor-alpha levels, total proteolytic activity, Porphyromonas gingivalis (Pg) prevalence, and neutrophil-protease 3 messenger RNA (mRNA) expression in patients with chronic periodontitis compared to healthy control patients. The aim of the present study is to expand this observation by considering the site level according to the presence of Pg. Methods: Microbiologic and gingival crevicular fluid samples were collected from patients with chronic periodontitis. Pg presence was evaluated by polymerase chain reaction and PAR(2) mRNA expression was evaluated by reverse-transcription polymerase chain reaction. Total proteolytic activity in the crevicular fluid was analyzed by using a specific substrate benzoylarginine nitroanilide, and the proinflammatory mediators IL-1 alpha, IL-6, IL-8, and tumor necrosis factor-alpha were analyzed by enzyme-linked immunosorbent assay. Results: In Pg-positive periodontal sites, the mean probing depth and clinical attachment level, the prevalence of bleeding on probing sites, and crevicular fluid volume were higher (P < 0.05) compared to Pg-negative sites. In addition, with the exception of IL-8, all other inflammatory mediators were positively (P < 0.05) associated with Pg presence. Pg presence was also positively associated with a higher proteolytic activity (P = 0.0037) and higher PAR(2) mRNA expression (P = 0.0271). Conclusions: We conclude that in chronic periodontitis, periodontal pockets presenting Pg show an upregulation of PAR(2) gene expression, and higher proinflammatory profile associated with advanced clinical destruction, therefore suggesting that Pg plays a pivotal role on PAR(2)-mediated periodontal inflammation in humans. J Periodontol 2011;82:1596-1601. (AU)

Processo FAPESP: 10/16605-0 - Efeito da terapia periodontal sobre a expressão de Receptor Ativado por Protease do tipo 2 (PAR-2) em pacientes com periodontite crônica
Beneficiário:Marinella Holzhausen Caldeira
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 07/50665-8 - Associação do receptor tipo 2 ativado por protease (PAR2) com a doença periodontal em humanos
Beneficiário:Marinella Holzhausen Caldeira
Linha de fomento: Auxílio à Pesquisa - Regular