Studies on the Expression of Fibroblast Growth Factor-2 from Odontoblast-like Cells

Autor(es)

Oliveira, Sandra H. P. ^[1] ; Santos, Vanessa A. C. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Dept Basic Sci, Pharmacol Lab, Sch Dent Aracatuba, BR-16018805 Sao Paulo - Brazil

^[2] Univ Estadual Paulista, Dept Pediat & Social Dent, Sch Dent Aracatuba, BR-16018805 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENDODONTICS; v. 37, n. 11, p. 1520-1524, NOV 2011.
• Citações Web of Science: 4

Resumo

Introduction: The goal of this study was to evaluate the mechanism involved in the expression of fibroblast growth factor-2 (FGF-2) by odontoblast-like cells (ODs) stimulated by lipopolysaccharide (LPS) via p42/44, p38, and PI3K. Methods: ODs (MDPC-23) were stimulated with LPS for 1, 6, and 24 hours. The FGF-2 expression was evaluated by reverse transcriptase-polymerase chain reaction and protein production by Western blot analysis. Cells were pretreated with dexamethasone (DEX), MK886 (MK), p42/44 inhibitor (PD98059, PD), p38 inhibitor (SB202190, SB), or PI3K inhibitor (wortmannin, Wort) and then stimulated with LPS (0.1 mu g/mL) for 1 hour. Results: LPS-stimulated ODs express FGF-2 in concentrations at 0.1, 1, 10, and 100 mu g/mL after 1 hour. DEX and MK were able to inhibit FGF-2 mRNA expression. PD, SB, and Wort also decreased expression. Conclusions: LPS-induced FGF-2 mRNA expression on ODs occurs via leukotriene production or cytokine and/or chemokine production activating p42/44, p38, and PI3K pathway. The data suggest that FGF-2 released by ODs might act as modulators of immune response mainly in the tissue repair. (J Endod 2011;37:1520-1524) (AU)

• Processo FAPESP: 06/50603-0 - Estudo dos mecanismos envolvidos na ativação dos fibroblastos gengivais de camundongos diabéticos com doença periodontal experimental
• Beneficiário: Sandra Helena Penha de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/51486-2 - Estudo dos mecanismos envolvidos na expressão de SCF e FGF-2 por odontoblastos estimulados por LPS: papel das vias de sinalização intracelulares p38, p42/44 e PI3K
• Beneficiário: Vanessa Aparecida Carvalho Santos
• Modalidade de apoio: Bolsas no Brasil - Doutorado