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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Studies on the Expression of Fibroblast Growth Factor-2 from Odontoblast-like Cells

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Author(s):
Oliveira, Sandra H. P. [1] ; Santos, Vanessa A. C. [2]
Total Authors: 2
Affiliation:
[1] Univ Estadual Paulista, Dept Basic Sci, Pharmacol Lab, Sch Dent Aracatuba, BR-16018805 Sao Paulo - Brazil
[2] Univ Estadual Paulista, Dept Pediat & Social Dent, Sch Dent Aracatuba, BR-16018805 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF ENDODONTICS; v. 37, n. 11, p. 1520-1524, NOV 2011.
Web of Science Citations: 4
Abstract

Introduction: The goal of this study was to evaluate the mechanism involved in the expression of fibroblast growth factor-2 (FGF-2) by odontoblast-like cells (ODs) stimulated by lipopolysaccharide (LPS) via p42/44, p38, and PI3K. Methods: ODs (MDPC-23) were stimulated with LPS for 1, 6, and 24 hours. The FGF-2 expression was evaluated by reverse transcriptase-polymerase chain reaction and protein production by Western blot analysis. Cells were pretreated with dexamethasone (DEX), MK886 (MK), p42/44 inhibitor (PD98059, PD), p38 inhibitor (SB202190, SB), or PI3K inhibitor (wortmannin, Wort) and then stimulated with LPS (0.1 mu g/mL) for 1 hour. Results: LPS-stimulated ODs express FGF-2 in concentrations at 0.1, 1, 10, and 100 mu g/mL after 1 hour. DEX and MK were able to inhibit FGF-2 mRNA expression. PD, SB, and Wort also decreased expression. Conclusions: LPS-induced FGF-2 mRNA expression on ODs occurs via leukotriene production or cytokine and/or chemokine production activating p42/44, p38, and PI3K pathway. The data suggest that FGF-2 released by ODs might act as modulators of immune response mainly in the tissue repair. (J Endod 2011;37:1520-1524) (AU)