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Evaluation of the effects of the lectin Artin M in the expression of tissue repair-related growth factors and inflammatory cytokines: in vitro study in rat macrophages and gingival fibroblast

Grant number: 12/13922-0
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2013
Effective date (End): February 28, 2014
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal Investigator:Joni Augusto Cirelli
Grantee:Fernanda Florian
Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil


Currently several studies in Periodontics and Implantology seek for new procedures and material that optimize the healing process. The healing process involves the proliferation of various cells that act under the coordination of proteins called growth factors and/or cytokines, which have been focused by many researches that have confirmed their special role in the repair process. Artim M is a lectin isolated from Artocarpus integrifolia seed and used in the topical treatment of skin injuries from burns providing accelerated healing and reduction of necrosis. In the oral cavity, our group demonstrated an increase in the in vivo induction of neutrophils and acceleration of healing. The aim of this study is to evaluate the effect of Artim M lectin on gene expression and protein production of growth factors (VEGF, FGF², TGF²) and inflammatory cytokines (IL-1, TNF±) by real-time polymerase chain reaction after reverse transcription (RT-qPCR) and quantitative analysis of concentration of those proteins in culture supernatants by ELISA (Enzyme linked Immunosorbent Assay). The cells will be obtained by primary culture of rat gingival fibroblast and of macrophages from bone narrow of long bones of rats in the Laboratory of Molecular Biology, Department of Periodontology, School of Dentistry of Araraquara, UNESP. In this study we expected to demonstrate a direct effect of Artim M in specific cells (gingival fibroblasts) involved in the gingival tissue repair, and macrophages that are responsible for secretion of proteins important for the repair process. These analyzes aim to better understand the effect of this new product in the healing process, obtaining data that will allow new studies in the application of Artin M in Periodontics and other areas.

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