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Androgens impact on the process of tissue repair and mechanism of bone resorption.

Abstract

The aim of the project will be evaluated in vivo the supra and sub physiological effects of testosterone, as well as pharmacological inhibition of androgen receptor, estrogen and aromatase enzyme on the tissue repair (wound healing and scarring), and on induced bone resorption using a model resorption by Porphyromonas gingivalis calvaria injection. It will be used 80 rats (Rattus norvegicus albinus Holtzman), male, approximately 2-3 months of age randomly divided into 8 experimental groups: G1-Control, G2-Testosterone, G3-Anastrozole, Flutamide-G4, G5-Fulvestrant, G6-Testosterone + Anastrozole, G7-Orchiectomy (OQX) and G8- OQX+Testosterone. The animals will be submitted to different therapies for regulating / hormonal deprivation for a period of 15 days, and then subjected to procedures for cutaneous wound induction (for 7 days) and induction of bone resorption (over a period of 6 days). Macro and microscopic analysis of cutaneous wounds will be made, evaluating the expression of growth factors and local inflammatory cytokines profile, and cell proliferation activity of the cutaneous tissue sample; and similarly, calvaria samples are submitted for microscopic evaluation, and analysis of the bone defect volume percentage and density of the bone surface using computerized microtomography analysis, and also, will be evaluate the inflammatory cytokines expression in the cutaneous tissue adjacent to the calvaria. The data obtained will be analyzed by statistical analysis using the software GraphPad Prism 6, and all tests of this study will be applied with a confidence level of 95% (p <0.05). (AU)