| Texto completo | |
| Autor(es): |
Okuro, Paula K.
;
Viau, Michele
;
Marze, Sebastien
;
Laurent, Sophie
;
Cunha, Rosiane L.
;
Berton-Carabin, Claire
;
Meynier, Anne
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FOOD & FUNCTION; v. 14, n. 24, p. 14-pg., 2023-11-21. |
| Resumo | |
Investigating the gastrointestinal fate of food emulsions is critical to unveil their nutritional relevance. To this end, the protocol standardized by COST INFOGEST 2.0 is meaningful for guiding in vitro digestion experiments. In contrast with studies addressing emulsions with low dispersed phase volume fraction (phi 0.05-0.1), we presently raise some points for a proper interpretation of the digestibility of emulsions with high lipid content using the pH-stat method. Oil-in-water high internal phase emulsions (HIPEs) were submitted to gastric pre-lipolysis with the addition of rabbit gastric lipase (RGE). Commercial mayonnaise (phi 0.76) was systematically diluted (phi 0.025, 0.05, 0.1, 0.15, 0.25, 0.4, and 0.76) to cover a wide range of enzyme-to-lipid ratios (8.5-0.3 U per mu mol for RGE and 565.1-18.6 U per mu mol for pancreatin, in the gastric and intestinal phases, respectively). Lipolysis was tracked either by fatty acid titration (NaOH titration) or completed by analysis of lipid classes and fatty acid composition. Gastric lipase resulted in substantial lipid hydrolysis, reaching 20 wt% at low lipid fractions (phi 0.025 and 0.05). Likewise, the kinetics and extent of lipolysis during intestinal digestion were modulated by the enzyme-to-substrate ratio. A logarithmic relationship between lipid hydrolysis and lipid concentration was observed, with a very limited extent at the highest lipid content (phi 0.76). A holistic interpretation relying on FFA titration and further evaluation of all lipolytic products appears of great relevance to capture the complexity of the effects involved. Overall, this work contributes to rationally and critically evaluating the outcomes of static in vitro experiments of lipid digestion. (AU) | |
| Processo FAPESP: | 19/27354-3 - Arquitetura de sistemas de liberação coloidais: qual é o papel da estrutura na digestibilidade? |
| Beneficiário: | Rosiane Lopes da Cunha |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 19/26046-3 - Avaliação de emulsões com alto carregamento de fase dispersa como estratégia para modulação da digestão lipídica in vitro e da bioacessibilidade de componente bioativo |
| Beneficiário: | Paula Kiyomi Okuro |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado |
| Processo FAPESP: | 18/20308-3 - Sistemas estruturados coloidais com base oleosa: comportamento da estrutura frente à digestibilidade e aumento da biodisponibilidade de bioativo |
| Beneficiário: | Paula Kiyomi Okuro |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |