Unveiling the peptidome diversity of Lachesis muta snake venom: Discovery of novel fragments of metalloproteinase, L-amino acid oxidase, and bradykinin potentiating peptides

Autor(es)

Ito, Lucas T. ; Miyamoto, Jackson G. ; Sant'Anna, Savio S. ; Grego, Kathleen F. ; Tanaka-Azevedo, Anita M. ; Tashima, Alexandre K.

• Tipo de documento: Artigo Científico
• Fonte: Biochemical and Biophysical Research Communications; v. 683, p. 5-pg., 2023-10-18.

Resumo

Snake venoms are known to be major sources of peptides with different pharmacological properties. In this study, we comprehensively explored the venom peptidomes of three specimens of Lachesis muta, the largest venomous snake in South America, using mass spectrometry techniques. The analysis revealed 19 main chromatographic peaks common to all specimens. A total of 151 peptides were identified, including 69 from a metalloproteinase, 58 from the BPP-CNP precursor, and 24 from a L-amino acid oxidase. To our knowledge, 126 of these peptides were reported for the first time in this work, including a new SVMP-derived peptide fragment, Lm-10a. Our findings highlight the dynamic nature of toxin maturation in snake venoms, driven by proteolytic processing, post-translational modifications, and cryptide formation. (AU)

• Processo FAPESP: 17/21052-0 - Sepse: mecanismos, alvos terapêuticos e epidemiologia
• Beneficiário: Reinaldo Salomão
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/20106-9 - Peptidômica dos venenos de serpentes e aranhas brasileiras
• Beneficiário: Alexandre Keiji Tashima
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 21/11707-4 - Avaliação de métodos alternativos in vitro à utilização de animais para os ensaios in vivo no estudo de venenos de serpentes: princípio dos 3R's
• Beneficiário: Anita Mitico Tanaka-Azevedo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/07629-8 - Sequenciamento completo de toxinas ricas em cisteínas do veneno da aranha Nephilengys cruentata
• Beneficiário: Lucas Toshio Ito
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica