| Texto completo | |
| Autor(es): Mostrar menos - |
Trevisan-Silva, Dilza
;
Cosenza-Contreras, Miguel
;
Oliveira, Ursula C.
;
Da Ros, Nancy
;
Andrade-Silva, Debora
;
Menezes, Milene C.
;
Oliveira, Ana Karina
;
Rosa, Jaqueline G.
;
Sachetto, Ana T. A.
;
Biniossek, Martin L.
;
Pinter, Niko
;
Santoro, Marcelo L.
;
Nishiyama Jr, Milton Y.
;
Schilling, Oliver
;
Serrano, Solange M. T.
Número total de Autores: 15
|
| Tipo de documento: | Artigo Científico |
| Fonte: | International Journal of Biological Macromolecules; v. 253, p. 17-pg., 2023-10-24. |
| Resumo | |
Snakebite envenomation is classified as a Neglected Tropical Disease. Bothrops jararaca venom induces kidney injury and coagulopathy. HF3, a hemorrhagic metalloproteinase of B. jararaca venom, participates in the envenomation pathogenesis. We evaluated the effects of HF3 in mouse kidney and blood plasma after injection in the thigh muscle, mimicking a snakebite. Transcriptomic analysis showed differential expression of 31 and 137 genes related to kidney pathology after 2 h and 6 h, respectively. However, only subtle changes were observed in kidney proteome, with differential abundance of 15 proteins after 6 h, including kidney injury markers. N-ter-minomic analysis of kidney proteins showed 420 proteinase-generated peptides compatible with meprin speci-ficity, indicating activation of host proteinases. Plasma analysis revealed differential abundance of 90 and 219 proteins, respectively, after 2 h and 6 h, including coagulation-cascade and complement-system components, and creatine-kinase, whereas a semi-specific search of N-terminal peptides indicated activation of endogenous proteinases. HF3 promoted host reactions, altering the gene expression and the proteolytic profile of kidney tissue, and inducing plasma proteome imbalance driven by changes in abundance and proteolysis. The overall response of the mouse underscores the systemic action of a hemorrhagic toxin that transcends local tissue damage and is related to known venom-induced systemic effects. (AU) | |
| Processo FAPESP: | 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular |
| Beneficiário: | Hugo Aguirre Armelin |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 17/23871-8 - Caracterização molecular dos efeitos in vivo da metaloprotease hemorrágica HF3: análises proteômicas do plasma e do tecido renal de camundongos |
| Beneficiário: | Dilza Trevisan Silva |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado |
| Processo FAPESP: | 13/13548-4 - Caracterização proteômica / glicoproteômica de venenos de serpentes do complexo Bothrops jararaca com ênfase no N-terminoma e N-glicoma de toxinas |
| Beneficiário: | Solange Maria de Toledo Serrano |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 17/00715-0 - Enzimas proteolíticas de venenos de serpentes disparam cascatas de eventos moleculares ainda desconhecidos |
| Beneficiário: | Dilza Trevisan Silva |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |