| Texto completo | |
| Autor(es): |
de Queiroga, Anna Paula Rocha
;
de Souza, Gabriela Freitas Pereira
;
Assane, Inacio Mateus
;
Messias, Thiago
;
Pilarski, Fabiana
;
Schloter, Michael
;
Salles Jr., Airton Gonsalves
;
Rath, Susanne
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES; v. 1246, p. 10-pg., 2024-08-28. |
| Resumo | |
This study concerns the synthesis of the florfenicol (FF) metabolites florfenicol amine (FFA), florfenicol alcohol (FFOH), and monochloroflorfenicol (FFCl), for their subsequent use as reference standards in On-line solid-phase extraction-ultra high-performance liquid chromatography-tandem mass spectrometry (SPE-UHPLC-MS/MS) analysis. The metabolites were characterized using 1 H and 13 C NMR, as well as HRMS, and their purities were confirmed by quantitative NMR to ensure analytical reliability. Validation of the developed analytical method showed that it presented acceptable performance, with linearity >0.99 for all the target analytes, accuracies within +/- 10 % of nominal concentrations, and intra- and inter-day precisions within 15 %. Application of this method to fillets from fish that had been treated with florfenicol (dose of 10 mg/kg bw daily) demonstrated its effectiveness in consistently detecting FF and its metabolites throughout the treatment. The results emphasized the utility of the method for enhancing pharmacokinetic and residue depletion research. The ability to precisely monitor the drug and its metabolites in treated fish provides important insights into florfenicol metabolism, laying the groundwork for further comprehensive profiling studies of metabolites in fish tissue. (AU) | |
| Processo FAPESP: | 21/08152-0 - Desafio do uso de medicamentos na piscicultura no contexto One Health: avaliação da eficácia, segurança e análise de risco |
| Beneficiário: | Jonas Augusto Rizzato Paschoal |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 19/22775-0 - Combinação de tianfenicol com florfenicol: farmacocinética e eficácia terapêutica no tratamento da Estreptococose em Tilápia do Nilo (Oreochromis niloticus) |
| Beneficiário: | Inácio Mateus Assane |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 22/00343-4 - Multicatálise envolvendo organofotocatálise e organocatálise baseada em biomassa: uma plataforma sustentável para o desenvolvimento de novas reações enantiosseletivas |
| Beneficiário: | Airton Goncalves Salles Junior |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 22/08804-0 - Consequências do uso de antimicrobianos na aquicultura na saúde humana e no ecossistema aquático |
| Beneficiário: | Anna Paula Rocha de Queiroga |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |