Sleep loss and acute drug abuse can induce DNA damage in multiple organs of mice

Autor(es)

Alvarenga, T. A. ^[1] ; Ribeiro, D. A. ^[2] ; Araujo, P. ^[1] ; Hirotsu, C. ^[1] ; Mazaro-Costa, R. ^[1] ; Costa, J. L. ^[3] ; Battisti, M. C. ^[1] ; Tufik, S. ^[1] ; Andersen, M. L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Psicobiol, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Biociencias, Santos - Brazil

^[3] Criminalist Inst, Instrumental Anal Lab, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: HUMAN & EXPERIMENTAL TOXICOLOGY; v. 30, n. 9, p. 1275-1281, SEP 2011.
• Citações Web of Science: 10

Resumo

The purpose of the present study was to characterize the genetic damage induced by paradoxical sleep deprivation (PSD) in combination with cocaine or ecstasy (3,4-methylenedioxymethamphetamine; MDMA) in multiple organs of male mice using the single cell gel (comet) assay. C57BL/6J mice were submitted to PSD by the platform technique for 72 hours, followed by drug administration and evaluation of DNA damage in peripheral blood, liver and brain tissues. Cocaine was able to induce genetic damage in the blood, brain and liver cells of sleep-deprived mice at the majority of the doses evaluated. Ecstasy also induced increased DNA migration in peripheral blood cells for all concentrations tested. Analysis of damaged cells by the tail moment data suggests that ecstasy is a genotoxic chemical at the highest concentrations tested, inducing damage in liver or brain cells after sleep deprivation in mice. Taken together, our results suggest that cocaine and ecstasy/MDMA act as potent genotoxins in multiple organs of mice when associated with sleep loss. (AU)

• Processo FAPESP: 09/01030-5 - Efeito da privação e restrição de sono na função reprodutiva de ratos machos
• Beneficiário: Tathiana Aparecida Fernandes de Alvarenga
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/50130-0 - Estudo do sistema nociceptivo de camundongos submetidos a restrição de sono durante o período neonatal
• Beneficiário: Paula Cristina Alves Araújo
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 98/14303-3 - Center for Sleep Studies
• Beneficiário: Sergio Tufik
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 10/50129-1 - Insuficiência renal crônica, restrição de sono e sildenafil: consequências renais, cardíacas e sexuais em um modelo animal
• Beneficiário: Camila Hirotsu
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto