| Texto completo | |
| Autor(es): |
de Almeida, Michelle Teixeira
;
Barbosa, Ana Paula
;
Bomfim, Camila Gasque
;
Visnardi, Aline Biazola
;
Vinces, Tania Churasacari
;
Ceroni, Alexandre
;
Durigon, Edison Luiz
;
Guzzo, Cristiane Rodrigues
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF IMMUNOLOGICAL METHODS; v. 522, p. 9-pg., 2023-09-27. |
| Resumo | |
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is an enveloped, plus-stranded RNA virus responsible for the Coronavirus Disease 2019 (COVID-19). Patients infected with COVID-19 may be asymptomatic or have symptoms ranging from mild manifestations to severe cases of the disease that could lead to death. The SARS-CoV-2 genome encodes 4 structural proteins, including the Spike protein (S), the Nucleocapsid protein (N), Membrane protein (M) and, the Envelope protein (E). The N protein forms a major component of the ribonucleoprotein complex within the virus particle and play a vital role in its transcription and replication. Nevertheless, the S protein was the most important protein in the development of vaccines against COVID-19. However, the decrease in number of registered immunizations against the disease and the rapid drop in neutralizing antibody titers together with looser preventive measures for virus transmission, favored the rapid appearance of new variants of concerns (VOCs) that primarily show mutations in the S protein. This fact makes the N protein a good candidate for the development of diagnostic tests, due to its stability, amino acid conservation, high immunogenicity, and the smaller likelihood of mutation. With the aim of developing a new diagnostic kit based on the N protein, we evaluated the humoral response in female Wistar rats against this target. Three constructions of the N protein were used to inoculate the animals: the full-length protein (Cfull), the N(NTD), and the C-terminal (CTD) portion of the protein. The immunizations induced the animal's immune response, with specific polyclonal IgG antibodies against the Cfull protein and its fragments. There were not nonspecific bind to the protein used as negative control. Anti-Cfull antibodies demonstrated high efficiency in binding to the NTD protein and the antibodies present in the anti-CTD and anti-NTD sera have recognized the Cfull protein, but they were not able to recognize the NTD and CTD proteins, respectively. Our results indicate an efficient protocol for obtaining high antibody titers against the N recombinant protein of SARS-CoV-2 and its fragments highlighting the Cfull protein, which can be used in the development of new diagnostic kits. (AU) | |
| Processo FAPESP: | 21/10577-0 - Centro de Pesquisa em Biologia de Bactérias e Bacteriófagos (CEPID B3) |
| Beneficiário: | Shaker Chuck Farah |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 19/21739-0 - Atividade antimicrobiana de proteínas secretadas por Xanthomonas citri contra microrganismos de interesse médico |
| Beneficiário: | Camila Gasque Bomfim |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 22/08730-7 - Biologia estrutural de proteínas aplicada na compreensão da sinalização c-di-GMP e de sistemas secretórios bacterianos |
| Beneficiário: | Cristiane Rodrigues Guzzo Carvalho |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 20/14914-8 - Caracterização de LIC_11920 e seu papel na sinalização c-di-GMP |
| Beneficiário: | Tania Geraldine Churasacari Vinces |
| Modalidade de apoio: | Bolsas no Brasil - Programa Capacitação - Treinamento Técnico |
| Processo FAPESP: | 19/00195-2 - Compreensão das redes de sinalização c-di-GMP e do sistema de secreção tipo II presentes no patógeno humano Leptospira interrogans Copenhageni |
| Beneficiário: | Cristiane Rodrigues Guzzo Carvalho |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 16/09047-8 - Genômica comparativa de toxinas bacterianas associadas ao sistema secretório do tipo IV |
| Beneficiário: | Robson Francisco de Souza |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 20/04680-0 - Desenho de diferentes fragmentos da proteína de superfície, spike, do SARS-CoV-2, para desenvolvimento de testes rápidos e de vacina |
| Beneficiário: | Cristiane Rodrigues Guzzo Carvalho |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |