Polyalthic Acid from Copaifera lucens Demonstrates Anticariogenic and Antiparasitic Properties for Safe Use

Autor(es):Mostrar menos -

Santiago, Mariana B. ; dos Santos, Vinicius Cristian O. ; Teixeira, Samuel C. ; Silva, Nagela B. S. ; de Oliveira, Pollyanna F. ; Ozelin, Saulo D. ; Furtado, Ricardo A. ; Tavares, Denise C. ; Ambrosio, Sergio Ricardo ; Veneziani, Rodrigo Cassio S. ; Ferro, Eloisa Amalia V. ; Bastos, Jairo K. ; Martins, Carlos Henrique G.

• Tipo de documento: Artigo Científico
• Fonte: PHARMACEUTICALS; v. 16, n. 10, p. 19-pg., 2023-10-01.

Resumo

This study aimed at evaluating the potential of Copaifera lucens, specifically its oleoresin (CLO), extract (CECL), and the compound ent-polyalthic acid (PA), in combating caries and toxoplasmosis, while also assessing its toxicity. The study involved multiple assessments, including determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against cariogenic bacteria. CLO and PA exhibited MIC and MBC values ranging from 25 to 50 mu g/mL, whereas CECL showed values equal to or exceeding 400 mu g/mL. PA also displayed antibiofilm activity with minimum inhibitory concentration of biofilm (MICB50) values spanning from 62.5 to 1000 mu g/mL. Moreover, PA effectively hindered the intracellular proliferation of Toxoplasma gondii at 64 mu g/mL, even after 24 h without treatment. Toxicological evaluations included in vitro tests on V79 cells, where concentrations ranged from 78.1 to 1250 mu g/mL of PA reduced colony formation. Additionally, using the Caenorhabditis elegans model, the lethal concentration (LC50) of PA was determined as 1000 mu g/mL after 48 h of incubation. Notably, no significant differences in micronucleus induction and the NDI were observed in cultures treated with 10, 20, or 40 mu g/mL of CLO. These findings underscore the safety profile of CLO and PA, highlighting their potential as alternative treatments for caries and toxoplasmosis. (AU)

• Processo FAPESP: 16/20589-7 - Copaifera lucens: ação antibacteriana do extrato bruto hidroalcoólico e da oleorresina frente a patógenos bucais e seu potencial citotóxico e genotóxico
• Beneficiário: Vinicius Cristian Oti dos Santos
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 11/13630-7 - Validação química e farmacológica de extratos e princípios ativos de espécies de Copaifera
• Beneficiário: Jairo Kenupp Bastos
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/23012-2 - Potencial antibacteriano e antivirulência do óleo-resina e substâncias isoladas de espécies de Copaifera spp. frente aos principais agentes periodontais
• Beneficiário: Sergio Ricardo Ambrosio
• Modalidade de apoio: Auxílio à Pesquisa - Regular