| Texto completo | |
| Autor(es): |
Alves, Ingrid R.
;
Vencio, Ricardo Z.
;
Galhardo, Rodrigo S.
Número total de Autores: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS; v. 825, p. 6-pg., 2022-07-01. |
| Resumo | |
UV-induced mutagenesis is, to greater extent, a phenomenon dependent on translesion synthesis (TLS) and regulated by the SOS response in bacteria. Caulobacter crescentus, like many bacterial species, employs the ImuABC (ImuAB DnaE2) pathway in TLS. To have a better understanding of the characteristics of UV-induced mutagenesis in this organism, we performed a whole genome analysis of mutations present in survivors after an acute UVC exposure (300 J/m(2)). We found an average of 3.2 mutations/genome in irradiated samples, distributed in a mutational spectrum consisting exclusively of base substitutions, including tandem mutations. Although limited in conclusions by the small number of mutations identified, our study points to the feasibility of using whole-genome sequencing to study mutagenesis occurring in experiments involving a single acute exposure to genotoxic agents. (AU) | |
| Processo FAPESP: | 09/51387-7 - Regulação da mutagênese no modelo de Caulobacter crescentus e suas implicações para a evolução bacteriana |
| Beneficiário: | Rodrigo da Silva Galhardo |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 19/19435-3 - Papel de danos no DNA e função mitocondrial em envelhecimento vascular, imune e neurológico (DNA MoVINg) |
| Beneficiário: | Carlos Frederico Martins Menck |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |