Lactobacillus sakei 1 and its bacteriocin influence adhesion of Listeria monocytogenes on stainless steel surface

Autor(es)

Winkelstroeter, Lizziane K. ^[1] ; Gomes, Bruna C. ^[1] ; Thomaz, Marta R. S. ^[1] ; Souza, Vanessa M. ^[1] ; De Martinis, Elaine C. P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FOOD CONTROL; v. 22, n. 8, p. 1404-1407, AUG 2011.
• Citações Web of Science: 39

Resumo

Listeria monocytogenes is of particular concern for the food industry due to its psychrotolerant and ubiquitous nature. In this work, the ability of L monocytogenes culturable cells to adhere to stainless steel coupons was studied in co-culture with the bacteriocin-producing food isolate Lactobacillus sakei 1 as well as in the presence of the cell-free neutralized supernatant of L sakei 1 (CFSN-S1) containing sakacin 1. Results were compared with counts obtained using a non bacteriocin-producing strain (L sakei ATCC 15521) and its bacteriocin free supernatant (CFSN-SA). Culturable adherent L monocytogenes and lactobacilli cells were enumerated respectively on PALCAM and MRS agars at 3-h intervals for up to 12 h and after 24 and 48 h of incubation. Bacteriocin activity was evaluated by critical dilution method. After 6 h of incubation, the number of adhered L monocytogenes cells in pure culture increased from 3.8 to 5.3 log CFU/cm(2) (48h). Co-culture with L sakei 1 decreased the number of adhered L monocytogenes cells (P < 0.001) during all sampling times with counts lower than 3.0 log CFU/cm(2). The CFNS-S1 also led to a significant and similar reduction in culturable adhered L. monocytogenes counts for up to 24 h of incubation, however after 48 h of incubation, re-growth of L monocytogenes number of adhered cells was observed, likely due to lack of competition for nutrients. L sakei ATCC 15521 or its supernatant (CFNS-SA) did not reduce the number of adhered L monocytogenes cells on stainless steel surface and from 6 h of incubation, listerial counts were between 4.3 and 4.5 log CFU/cm(2). These results indicate that L sakei 1 and its bacteriocin sakacin 1 may be useful to inhibit early stages of L monocytogenes adherence to abiotic surface. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 07/56720-0 - Formacao de biofilmes por listeria monocytogenes: fatores de estresse e avaliacao de viabilidade celular.
• Beneficiário: Elaine Cristina Pereira de Martinis
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/56365-6 - Expressão de proteínas fluorescentes em Lactobacillus sakei 1 bacteriocinogênico e Listeria monocytogenes para visualização da capacidade de adesão em células Caco-2 na presença de prebióticos e, resistência de L. sakei 1 frente a condições que simulem o trato gastrointestinal de humanos
• Beneficiário: Bruna Carrer Gomes Malavazi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 06/05574-1 - Aplicacao de reacao da polimerase em cadeia em tempo real para a avaliacao da formacao de biofilmes por listeria monocytogenes.
• Beneficiário: Lizziane Kretli Winkelströter Eller
• Modalidade de apoio: Bolsas no Brasil - Mestrado