Aerobic Exercise Improves Reverse Cholesterol Transport in Cholesteryl Ester Transfer Protein Transgenic Mice

Autor(es)

Rocco, D. D. F. M. ^[1] ; Okuda, L. S. ^[1] ; Pinto, R. S. ^[1] ; Ferreira, F. D. ^[1] ; Kubo, S. K. ^[2] ; Nakandakare, E. R. ^[1] ; Quintao, E. C. R. ^[1] ; Catanozi, S. ^[1] ; Passarelli, M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Lipids Lab LIM 10, Fac Med Sci, BR-01246000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Emergency Care Res Unit LIM 51, Fac Med Sci, BR-01246000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: LIPIDS; v. 46, n. 7, p. 617-625, JUL 2011.
• Citações Web of Science: 15

Resumo

We analyzed the effect of a 6-week aerobic exercise training program on the in vivo macrophage reverse cholesterol transport (RCT) in human cholesteryl ester transfer protein (CETP) transgenic (CETP-tg) mice. Male CETP-tg mice were randomly assigned to a sedentary group or a carefully supervised exercise training group (treadmill 15 m/min, 30 min sessions, five sessions per week). The levels of plasma lipids were determined by enzymatic methods, and the lipoprotein profile was determined by fast protein liquid chromatography (FPLC). CETP activity was determined by measuring the transfer rate of (14)C-cholesterol from HDL to apo-B containing lipoproteins, using plasma from CETP-tg mice as a source of CETP. The reverse cholesterol transport was determined in vivo by measuring the {[}(3)H]-cholesterol recovery in plasma and feces (24 and 48 h) and in the liver (48 h) following a peritoneal injection of {[}(3)H]-cholesterol labeled J774-macrophages into both sedentary and exercise trained mice. The protein levels of liver receptors were determined by immunoblot, and the mRNA levels for liver enzymes were measured using RT-PCR. Exercise training did not significantly affect the levels of plasma lipids or CETP activity. The HDL fraction assessed by FPLC was higher in exercise-trained compared to sedentary mice. In comparison to the sedentary group, a greater recovery of {[}(3)H]-cholesterol from the injected macrophages was found in the plasma, liver and feces of exercise-trained animals. The latter occurred even with a reduction in the liver CYP7A1 mRNA level in exercised trained animals. Exercise training increased the liver LDL receptor and ABCA-1 protein levels, although the SR-BI protein content was unchanged. The RCT benefit in CETP-tg mice elicited by exercise training helps to elucidate the role of exercise in the prevention of atherosclerosis in humans. (AU)

• Processo FAPESP: 07/56654-8 - Secreção de mediadores inflamatórios e transporte reverso de colesterol em macrófagos submetidos a glicoxidação
• Beneficiário: Ligia Shimabukuro Okuda
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 06/52702-5 - Influência do treinamento físico aeróbio sobre o transporte reverso do colesterol em camundongos C57Bl/6J selvagens e trangênicos para CETP humana
• Beneficiário: Débora Dias Ferraretto Moura Rocco
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 09/53412-9 - Estresse glicoxidativo e fluxo de lípides em macrófagos: papel dos inibidores de glicação avançada
• Beneficiário: Raphael de Souza Pinto
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 07/50387-8 - Influência do treinamento físico aeróbio sobre o transporte reverso de colesterol em camundongos C57Bl/6J selvagens e transgênicos para CETP humana
• Beneficiário: Marisa Passarelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular