Highly virulent colistin-susceptible Salmonella Havana ST1524 carrying the mcr-9.1 gene in food

Autor(es)

Monte, Daniel F. M. ; Fuga, Bruna ; Pariona, Jesus G. M. ; Vasquez-Ponce, Felipe ; Becerra, Johana ; Keelara, Shivaramu ; Thakur, Siddhartha ; Fedorka-Cray, Paula ; Lincopan, Nilton ; Landgraf, Mariza

• Tipo de documento: Artigo Científico
• Fonte: Microbial Pathogenesis; v. 199, p. 6-pg., 2024-12-27.

Resumo

Salmonella enterica subsp. enterica serovar Havana is a potential pathogenic serotype that can cause human foodborne illness. Therefore, we have conducted a microbiological and genomic surveillance study of Salmonella Havana from food in Brazil. Strikingly, we identified a colistin-susceptible Salmonella Havana strain (NC_STy4) carrying the mcr-9 gene, in salted chicken breast. Genomic analysis revealed that Salmonella Havana strain belonged to the international sequence type (ST) ST1524, circulating at the human-animal-environmental interface. Although the resistome analysis confirmed the presence of mcr-9 and aac(6')-Iaa genes, as well as a parC (Thr57 -> Ser) mutation, this strain exhibited a pansuceptible phenotype. Noteworthy, Salmonella Havana NC_STy4 exhibited a highly virulent behavior, killing 100 % of Galleria mellonella larvae at 13 h post-infection. In this regard, invasins (invA, ibeB), non-fimbrial adherence (misL), fimbrial adherence (fimH), magnesium uptake (mgtB and mgtC), regulation (phoP, phoQ, and pipB), secretion system (ssaHJKLNOQRU), and translocated effector (sopB/sigD and sopE2) virulence genes were predicted. IncHI2 and IncHI2A plasmids were also identified. While these findings underscore the urgent need for surveillance and monitoring foodborne pathogens carrying a wide virulome, colistin-susceptible Salmonella isolates may be silent reservoirs of the mcr genes. (AU)

• Processo FAPESP: 16/03044-7 - Diversidade genética, genes de virulência e resistência antimicrobiana de isolados de Salmonella spp. provenientes de abatedouros de frango
• Beneficiário: Daniel Farias Marinho Do Monte
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/07914-8 - FoRC - Centro de Pesquisa em Alimentos
• Beneficiário: Bernadette Dora Gombossy de Melo Franco
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 17/15967-5 - Abordagem de sequenciamento do genoma completo para estudar Salmonella enterica multirresistentes isoladas de abatedouros do Brasil
• Beneficiário: Daniel Farias Marinho Do Monte
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado