| Texto completo | |
| Autor(es): |
Monte, Daniel F. M.
;
Fuga, Bruna
;
Pariona, Jesus G. M.
;
Vasquez-Ponce, Felipe
;
Becerra, Johana
;
Keelara, Shivaramu
;
Thakur, Siddhartha
;
Fedorka-Cray, Paula
;
Lincopan, Nilton
;
Landgraf, Mariza
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Microbial Pathogenesis; v. 199, p. 6-pg., 2024-12-27. |
| Resumo | |
Salmonella enterica subsp. enterica serovar Havana is a potential pathogenic serotype that can cause human foodborne illness. Therefore, we have conducted a microbiological and genomic surveillance study of Salmonella Havana from food in Brazil. Strikingly, we identified a colistin-susceptible Salmonella Havana strain (NC_STy4) carrying the mcr-9 gene, in salted chicken breast. Genomic analysis revealed that Salmonella Havana strain belonged to the international sequence type (ST) ST1524, circulating at the human-animal-environmental interface. Although the resistome analysis confirmed the presence of mcr-9 and aac(6')-Iaa genes, as well as a parC (Thr57 -> Ser) mutation, this strain exhibited a pansuceptible phenotype. Noteworthy, Salmonella Havana NC_STy4 exhibited a highly virulent behavior, killing 100 % of Galleria mellonella larvae at 13 h post-infection. In this regard, invasins (invA, ibeB), non-fimbrial adherence (misL), fimbrial adherence (fimH), magnesium uptake (mgtB and mgtC), regulation (phoP, phoQ, and pipB), secretion system (ssaHJKLNOQRU), and translocated effector (sopB/sigD and sopE2) virulence genes were predicted. IncHI2 and IncHI2A plasmids were also identified. While these findings underscore the urgent need for surveillance and monitoring foodborne pathogens carrying a wide virulome, colistin-susceptible Salmonella isolates may be silent reservoirs of the mcr genes. (AU) | |
| Processo FAPESP: | 16/03044-7 - Diversidade genética, genes de virulência e resistência antimicrobiana de isolados de Salmonella spp. provenientes de abatedouros de frango |
| Beneficiário: | Daniel Farias Marinho Do Monte |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 13/07914-8 - FoRC - Centro de Pesquisa em Alimentos |
| Beneficiário: | Bernadette Dora Gombossy de Melo Franco |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 17/15967-5 - Abordagem de sequenciamento do genoma completo para estudar Salmonella enterica multirresistentes isoladas de abatedouros do Brasil |
| Beneficiário: | Daniel Farias Marinho Do Monte |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |