Changes in the intestinal microbiota induced by the postnatal environment and their association with hypertension

Autor(es)

Dardi, Patrizia ; Coutinho, Camille Perella ; de Oliveira, Sarah ; Teixeira, Simone Aparecida ; Gacek, Renaide Rodrigues Ferreira ; Purgatto, Eduardo ; Vinolo, Marco Aurelio Ramirez ; Muscara, Marcelo Nicolas ; Rossoni, Luciana Venturini

• Tipo de documento: Artigo Científico
• Fonte: PHARMACOLOGICAL RESEARCH; v. 212, p. 16-pg., 2025-01-25.

Resumo

It has been established that cross-fostering impacts the development of hypertension in spontaneously hypertensive rats (SHR). However, the ability of the cross-fostering protocol to shape gut microbiota profile in SHR and impact hypertension is not known. In this sense, the current study explored the influence of normotensive and hypertensive postnatal environments on the intestinal microbiota structure, composition, and functional capacity of SHR and Wistar rats. Our findings revealed significant differences in the microbiota's composition and its metabolic activity in young non-fostered SHR (SS) vs. Wistar (WW) rats, even before hypertension onset, characterized by a reduction of the "low-abundance" bacterial genera, a diminished availability of fecal butyrate and elevated hydrogen sulfide (H2S) production by the SS gut microbiota. Despite influencing the microbiota of both strains, cross-fostering did not fully replicate the microbiota composition of the naturally reared groups in the SHR nursed by Wistar mothers (SW), or in the Wistar rats breastfed by SHR mothers (WS). The SW group had fewer significant genera identified at the Partial Least Squares Discriminant Analysis (PLS-DA), despite resembling the genera profile identified in the normotensive group. While sharing bacterial genera with both SS and WW groups, the WS group is distinguished by its unique microbial composition, particularly by a greater diversity of the 'low-abundance' bacterial genera. Moreover, decreased systolic blood pressure was observed in the SW group compared to the SS group in adulthood. Thus, we could establish a link between microbiota composition and hypertension development, associating it with the loss of the "low-abundance" bacterial taxa. Our data suggest that the postnatal environment is pivotal to promoting gut microbiota compositional changes and contributes to hypertension development. (AU)

• Processo FAPESP: 20/10381-5 - Estudo do efeito da amamentação cruzada sobre as modificações intestinais que influenciam o remodelamento vascular e o desenvolvimento da Hipertensão Arterial
• Beneficiário: Patrizia Dardi
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/15313-8 - Análise dos mecanismos moleculares envolvidos na interação de metabólitos da microbiota e células do hospedeiro durante a inflamação
• Beneficiário: Marco Aurélio Ramirez Vinolo
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2
• Processo FAPESP: 23/03813-4 - Participação do Receptor de Mineralocorticoide na Disfunção do Tecido Adiposo Perivascular na Hipertensão Renovascular.
• Beneficiário: Luciana Venturini Rossoni
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 23/13436-3 - Estudo dos efeitos do doador mitocondrial de sulfeto de hidrogênio (H2S) AP39 sobre células endoteliais e musculares lisas vasculares em cultura.
• Beneficiário: Marcelo Nicolas Muscara
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/08026-5 - Ajustes da inervação perivascular e do remodelamento em artéria de resistência do modelo de hipertensão arterial DOCA-sal: participação da ouabaína endógena
• Beneficiário: Luciana Venturini Rossoni
• Modalidade de apoio: Auxílio à Pesquisa - Regular