CO2 from Alcoholic Fermentation for Continuous Cultivation of Arthrospira (Spirulina) platensis in Tubular Photobioreactor Using Urea as Nitrogen Source

Autor(es)

Matsudo, Marcelo C. ^[1] ; Bezerra, Raquel P. ^{[2, 1]} ; Converti, Attilio ^[2] ; Sato, Sunao ^[1] ; Carvalho, Joao Carlos M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Pharmaceut Technol & Biochem, Fac Pharmaceut Sci, BR-05508900 Sao Paulo - Brazil

^[2] Univ Genoa, Dept Chem & Proc Engn GB Bonino, I-16145 Genoa - Italy

• Tipo de documento: Artigo Científico
• Fonte: BIOTECHNOLOGY PROGRESS; v. 27, n. 3, p. 650-656, MAY-JUN 2011.
• Citações Web of Science: 13

Resumo

Carbon dioxide released from alcoholic fermentation accounts for 33% of the whole CO(2) involved in the use of ethanol as fuel derived from glucose. As Arthrospira platensis can uptake this greenhouse gas, this study evaluates the use of the CO(2) released from alcoholic fermentation for the production of Arthrospira platensis. For this purpose, this cyanobacterium was cultivated in continuous process using urea as nitrogen source, either using CO(2) from alcoholic fermentation, without any treatment, or using pure CO(2) from cylinder. The experiments were carried out at 120 mu mol photons m(-2) s(-1) in tubular photobioreactor at different dilution rates (0.2 <= D <= 0.8 d(-1)). Using CO(2) from alcoholic fermentation, maximum steady-state cell concentration (2661 +/- 71 mg L(-1)) was achieved at D 0.2 d(-1), whereas higher dilution rate (0.6 d(-1)) was needed to maximize cell productivity (839 mg L(-1) d(-1)). This value was 10% lower than the one obtained with pure CO(2), and there was no significant difference in the biomass protein content. With D 0.8 d(-1), it was possible to obtain 56% +/- 1.5% and 50% +/- 1.2% of protein in the dry biomass, using pure CO(2) and CO(2) from alcoholic fermentation, respectively. These results demonstrate that the use of such cost free CO(2) from alcoholic fermentation as carbon source, associated with low cost nitrogen source, may be a promising way to reduce costs of continuous cultivation of photosynthetic microorganisms, contributing at the same time to mitigate the greenhouse effect. (C) 2011 American Institute of Chemical Engineers Biotechnol. Prog., 27: 650-656, 2011 (AU)

• Processo FAPESP: 06/54960-1 - Cultivo continuo de spirulina platensis (arthrospira) em reator tubular utilizando ureia como fonte de nitrogenio e co2 puro ou proveniente de fermentacao alcoolica.
• Beneficiário: Marcelo Chuei Matsudo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 06/56976-2 - Cultivo de Spirulina platensis (Arthrospira) em reator tubular utilizando ureia como fonte de nitrogênio e CO2 puro ou proveniente de fermentação alcoólica
• Beneficiário: João Carlos Monteiro de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Regular