Angiotensin I-converting enzyme (ACE) activity and expression in rat central nervous system after sleep deprivation

Autor(es)

Visniauskas, Bruna ^[1] ; Oliveira, Vitor ^[2] ; Carmona, Adriana K. ^[2] ; D'Almeida, Vania ^[1] ; de Melo, Robson L. ^[3] ; Tufik, Sergio ^[1] ; Chagas, Jair R. ^{[1, 4]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo UNIFESP, Dept Psicobiol, BR-04024002 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo UNIFESP, Dept Biofis, BR-04039032 Sao Paulo - Brazil

^[3] Butantan Inst, Ctr Appl Toxinol CAT CEPID, BR-05503900 Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo UNIFESP, Dept Ciencias Saude, BR-11060001 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Biological Chemistry; v. 392, n. 6, p. 547-553, APR 2011.
• Citações Web of Science: 2

Resumo

Proteases are essential either for the release of neuropeptides from active or inactive proteins or for their inactivation. Neuropeptides have a fundamental role in sleep-wake cycle regulation and their actions are also likely to be regulated by proteolytic processing. Using fluorescence resonance energy transfer substrates, specific protease inhibitors and real-time PCR we demonstrate changes in angiotensin I-converting enzyme (ACE) expression and proteolytic activity in the central nervous system in an animal model of paradoxical sleep deprivation during 96 h (PSD). Male rats were distributed into five groups (PSD, 24 h, 48 h and 96 h of sleep recovery after PSD and control). ACE activity and mRNA levels were measured in hypothalamus, hippocampus, brainstem, cerebral cortex and striatum tissue extracts. In the hypothalamus, the significant decrease in activity and mRNA levels, after PSD, was only totally reversed after 96 h of sleep recovery. In the brainstem and hippocampus, although significant, changes in mRNA do not parallel changes in ACE specific activity. Changes in ACE activity could affect angiotensin II generation, angiotensin 1-7, bradykinin and opioid peptides metabolism. ACE expression and activity modifications are likely related to some of the physiological changes (cardiovascular, stress, cognition, metabolism function, water and energy balance) observed during and after sleep deprivation. (AU)

• Processo FAPESP: 98/14303-3 - Center for Sleep Studies
• Beneficiário: Sergio Tufik
• Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs