Adipose Tissue-Derived Stem Cells from Humans and Mice Differ in Proliferative Capacity and Genome Stability in Long-Term Cultures

Autor(es)

Danoviz, Maria Elena ; Bassaneze, Vinicius ; Nakamuta, Juliana Sanajotti ; dos Santos-Junior, Gabriel Ribeiro ; Saint-Clair, Danilo ; Bajgelman, Marcio Chaim ; Fae, Kellen Cristhina ; Kalil, Jorge ; Miyakawa, Ayumi Aurea ; Krieger, Jose Eduardo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Heart Inst InCor, Lab Genet Mol Cardiol, LIM 13, BR-05403000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: STEM CELLS AND DEVELOPMENT; v. 20, n. 4, p. 661-670, APR 2011.
• Citações Web of Science: 19

Resumo

Adipose tissue-derived stem cells (ASCs) are among the more attractive adult stem cell options for potential therapeutic applications. Here, we studied and compared the basic biological characteristics of ASCs isolated from humans (hASCs) and mice (mASCs) and maintained in identical culture conditions, which must be examined prior to considering further potential clinical applications. hASCs and mASCs were compared for immunophenotype, differentiation potential, cell growth characteristics, senescence, nuclear morphology, and DNA content. Although both strains of ASCs displayed a similar immunophenotype, the percentage of CD73(+) cells was markedly lower and CD31(+) was higher in mASC than in hASC cultures. The mean population doubling time was 98.08 +/- 6.15 h for hASCs and 52.58 +/- 3.74 h for mASCs. The frequency of nuclear aberrations was noticeably lower in hASCs than in mASCs regardless of the passage number. Moreover, as the cells went through several in vitro passages, mASCs showed changes in DNA content and cell cycle kinetics (frequency of hypodiploid, G0/G1, G2/M, and hyperdiploid cells), whereas all of these parameters remained constant in hASCs. Collectively, these results suggest that mASCs display higher proliferative capacity and are more unstable than hASCs in long-term cultures. These results underscore the need to consider specificities among model systems that may influence outcomes when designing potential human applications. (AU)

• Processo FAPESP: 05/57591-4 - Caracterizacao da influencia do shear stress na diferenciacao de celulas mesenquimais de tecido adiposo em celulas endoteliais.
• Beneficiário: Vinícius Bassaneze
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 05/54695-3 - Reparação cardíaca biológica pós-infarto: uso de células tronco derivadas do tecido adiposo, isoladas ou associadas a fibroblastos a fibroblastos geneticamente modificados para produzir VEGF
• Beneficiário: Maria Elena Danoviz
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 04/06784-4 - Capacidade de transdiferenciação das células mesenquimais do tecido adiposo: estudo no reparo cardiovascular
• Beneficiário: Juliana Sanajotti Nakamuta
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 06/57937-0 - Estudo do crescimento populacional das celulas mesenquimais de tecido adiposo: implicacoes no fenotipo celular.
• Beneficiário: Gabriel Ribeiro dos Santos Júnior
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica