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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Adipose Tissue-Derived Stem Cells from Humans and Mice Differ in Proliferative Capacity and Genome Stability in Long-Term Cultures

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Author(s):
Danoviz, Maria Elena ; Bassaneze, Vinicius ; Nakamuta, Juliana Sanajotti ; dos Santos-Junior, Gabriel Ribeiro ; Saint-Clair, Danilo ; Bajgelman, Marcio Chaim ; Fae, Kellen Cristhina ; Kalil, Jorge ; Miyakawa, Ayumi Aurea ; Krieger, Jose Eduardo [1]
Total Authors: 10
Affiliation:
[1] Univ Sao Paulo, Sch Med, Heart Inst InCor, Lab Genet Mol Cardiol, LIM 13, BR-05403000 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: STEM CELLS AND DEVELOPMENT; v. 20, n. 4, p. 661-670, APR 2011.
Web of Science Citations: 19
Abstract

Adipose tissue-derived stem cells (ASCs) are among the more attractive adult stem cell options for potential therapeutic applications. Here, we studied and compared the basic biological characteristics of ASCs isolated from humans (hASCs) and mice (mASCs) and maintained in identical culture conditions, which must be examined prior to considering further potential clinical applications. hASCs and mASCs were compared for immunophenotype, differentiation potential, cell growth characteristics, senescence, nuclear morphology, and DNA content. Although both strains of ASCs displayed a similar immunophenotype, the percentage of CD73(+) cells was markedly lower and CD31(+) was higher in mASC than in hASC cultures. The mean population doubling time was 98.08 +/- 6.15 h for hASCs and 52.58 +/- 3.74 h for mASCs. The frequency of nuclear aberrations was noticeably lower in hASCs than in mASCs regardless of the passage number. Moreover, as the cells went through several in vitro passages, mASCs showed changes in DNA content and cell cycle kinetics (frequency of hypodiploid, G0/G1, G2/M, and hyperdiploid cells), whereas all of these parameters remained constant in hASCs. Collectively, these results suggest that mASCs display higher proliferative capacity and are more unstable than hASCs in long-term cultures. These results underscore the need to consider specificities among model systems that may influence outcomes when designing potential human applications. (AU)