| Texto completo | |
| Autor(es): |
Guilhen Longo, Larissa Valle
;
Breyer, Carlos Alexandre
;
Novaes, Gabriela Machado
;
Gegembauer, Gregory
;
Leitao Jr, Natanael Pinheiro
;
Octaviano, Carla Elizabete
;
Toyama, Marcos Hikari
;
de Oliveira, Marcos Antonio
;
Puccia, Rosana
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY; v. 10, p. 12-pg., 2020-08-04. |
| Resumo | |
Paracoccidioides brasiliensisis a temperature-dependent dimorphic fungus that causes systemic paracoccidioidomycosis, a granulomatous disease. The massive production of reactive oxygen species (ROS) by the host's cellular immune response is an essential strategy to restrain the fungal growth. Among the ROS, the hydroperoxides are very toxic antimicrobial compounds and fungal peroxidases are part of the pathogen neutralizing antioxidant arsenal against the host's defense. Among them, the peroxiredoxins are highlighted, since some estimates suggest that they are capable of decomposing most of the hydroperoxides generated in the host's mitochondria and cytosol. We presently characterized a uniqueP. brasiliensis1-Cys peroxiredoxin (PbPrx1). Our results reveal that it can decompose hydrogen peroxide and organic hydroperoxides very efficiently. We showed that dithiolic, but not monothiolic compounds or heterologous thioredoxin reductant systems, were able to retain the enzyme activity. Structural analysis revealed that PbPrx1 has an alpha/beta structure that is similar to the 1-Cys secondary structures described to date and that the quaternary conformation is represented by a dimer, independently of the redox state. We investigated the PbPrx1 localization using confocal microscopy, fluorescence-activated cell sorter, and immunoblot, and the results suggested that it localizes both in the cytoplasm and at the cell wall of the yeast and mycelial forms ofP. brasiliensis, as well as in the yeast mitochondria. Our present results point to a possible role of this uniqueP. brasiliensis1-Cys Prx1 in the fungal antioxidant defense mechanisms. (AU) | |
| Processo FAPESP: | 07/50930-3 - Análise funcional e estrutural de proteínas antioxidantes dependentes de tióis: uma investigação de mecanismos moleculares de catálise e da formação de complexos protéicos contendo dissulfetos mistos |
| Beneficiário: | Marcos Antonio de Oliveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 06/05095-6 - Análise de estruturas vesiculares exportadas e de moléculas secretadas pelos fungos patogênicos Paracoccidioides brasiliensis e Cryptococcus neoformans |
| Beneficiário: | Rosana Puccia |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 13/07937-8 - Redoxoma |
| Beneficiário: | Ohara Augusto |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 13/25950-1 - Expressão e regulação de genes relacionados à parede celular de fungos dimórficos patogênicos sob condições de estresse |
| Beneficiário: | Rosana Puccia |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 11/13500-6 - Investigação dos determinantes moleculares envolvidos na interação com os substratos de Tsa1 e Tsa2 de Saccharomyces cerevisiae |
| Beneficiário: | Carlos Alexandre Breyer |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 17/19942-7 - Busca de inibidores do sistema peroxirredoxina de patógenos e humanos |
| Beneficiário: | Marcos Antonio de Oliveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 17/20291-0 - Caracterização da atividade pró-inflamatória de uma nova serino protease (cdtsp-2) purificada do veneno total de Crotalus durissus terrificus |
| Beneficiário: | Marcos Hikari Toyama |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |