Interleukin-8 expression associated with canine mammary tumors

Autor(es)

Zuccari, D. A. P. C. ^{[1, 2]} ; Castro, R. ^[2] ; Gelaleti, G. B. ^[1] ; Mancini, U. M. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Programa Posgrad Genet, Sao Jose Do Rio Preto, SP - Brazil

^[2] Fac Med Sao Jose do Rio Preto, Programa Posgrad Ciencias Saude, Sao Jose Do Rio Preto, SP - Brazil

^[3] Univ Sao Paulo, Inst Biociencias, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Genetics and Molecular Research; v. 10, n. 3, p. 1522-1532, 2011.
• Citações Web of Science: 7

Resumo

The use of prognostic markers for mammary cancer is important for routine diagnosis and research. Interleukin-8 (IL-8) is a chemotactic cytokine, produced by several cell types in response to inflammation. The expression, regulation and function of IL-8 in dogs are little known. Recent studies have associated angiogenesis and inflammatory processes with tumor malignancy. We investigated a possible correlation between IL-8 expression and mammary tumor prognosis in female dogs. IL-8 expression was measured in 50 dogs with mammary neoplasia by immunohistochemistry and real-time PCR. Immunohistochemical staining was done with anti-IL-8 antibodies and PCR amplifications were performed in a 7500 Fast Real-Time PCR system. Gene expression stability was analyzed by the geNorm software. Quantitative real-time PCR showed that IL-8 expression decreased in malignant mammary cells compared to normal mammary tissue, while weak immunostaining was associated with a diagnosis of carcinoma. Complementing earlier studies on IL-8 expression in several types of cancer, including mammary cancer, we conclude that IL-8 has potential for use as a prognostic marker for canine mammary neoplasia. (AU)

• Processo FAPESP: 05/51936-0 - Avaliação imuno-histoquímica e molecular dos genes caveolina-1, interleucina-8, laminina-5 e serpin como marcadores prognósticos e preditivos nas neoplasias mamárias em cadelas
• Beneficiário: Debora Aparecida Pires de Campos Zuccari
• Modalidade de apoio: Auxílio à Pesquisa - Regular