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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Interleukin-8 expression associated with canine mammary tumors

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Autor(es):
Zuccari, D. A. P. C. [1, 2] ; Castro, R. [1] ; Gelaleti, G. B. [2] ; Mancini, U. M. [3]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Fac Med Sao Jose do Rio Preto, Programa Posgrad Ciencias Saude, Sao Jose Do Rio Preto, SP - Brazil
[2] Univ Estadual Paulista, Programa Posgrad Genet, Sao Jose Do Rio Preto, SP - Brazil
[3] Univ Sao Paulo, Inst Biociencias, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Genetics and Molecular Research; v. 10, n. 3, p. 1522-1532, 2011.
Citações Web of Science: 7
Resumo

The use of prognostic markers for mammary cancer is important for routine diagnosis and research. Interleukin-8 (IL-8) is a chemotactic cytokine, produced by several cell types in response to inflammation. The expression, regulation and function of IL-8 in dogs are little known. Recent studies have associated angiogenesis and inflammatory processes with tumor malignancy. We investigated a possible correlation between IL-8 expression and mammary tumor prognosis in female dogs. IL-8 expression was measured in 50 dogs with mammary neoplasia by immunohistochemistry and real-time PCR. Immunohistochemical staining was done with anti-IL-8 antibodies and PCR amplifications were performed in a 7500 Fast Real-Time PCR system. Gene expression stability was analyzed by the geNorm software. Quantitative real-time PCR showed that IL-8 expression decreased in malignant mammary cells compared to normal mammary tissue, while weak immunostaining was associated with a diagnosis of carcinoma. Complementing earlier studies on IL-8 expression in several types of cancer, including mammary cancer, we conclude that IL-8 has potential for use as a prognostic marker for canine mammary neoplasia. (AU)

Processo FAPESP: 05/51936-0 - Avaliação imuno-histoquímica e molecular dos genes caveolina-1, interleucina-8, laminina-5 e serpin como marcadores prognósticos e preditivos nas neoplasias mamárias em cadelas
Beneficiário:Debora Aparecida Pires de Campos Zuccari
Linha de fomento: Auxílio à Pesquisa - Regular