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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Interleukin-8 expression associated with canine mammary tumors

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Author(s):
Zuccari, D. A. P. C. [1, 2] ; Castro, R. [2] ; Gelaleti, G. B. [1] ; Mancini, U. M. [3]
Total Authors: 4
Affiliation:
[1] Univ Estadual Paulista, Programa Posgrad Genet, Sao Jose Do Rio Preto, SP - Brazil
[2] Fac Med Sao Jose do Rio Preto, Programa Posgrad Ciencias Saude, Sao Jose Do Rio Preto, SP - Brazil
[3] Univ Sao Paulo, Inst Biociencias, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Genetics and Molecular Research; v. 10, n. 3, p. 1522-1532, 2011.
Web of Science Citations: 7
Abstract

The use of prognostic markers for mammary cancer is important for routine diagnosis and research. Interleukin-8 (IL-8) is a chemotactic cytokine, produced by several cell types in response to inflammation. The expression, regulation and function of IL-8 in dogs are little known. Recent studies have associated angiogenesis and inflammatory processes with tumor malignancy. We investigated a possible correlation between IL-8 expression and mammary tumor prognosis in female dogs. IL-8 expression was measured in 50 dogs with mammary neoplasia by immunohistochemistry and real-time PCR. Immunohistochemical staining was done with anti-IL-8 antibodies and PCR amplifications were performed in a 7500 Fast Real-Time PCR system. Gene expression stability was analyzed by the geNorm software. Quantitative real-time PCR showed that IL-8 expression decreased in malignant mammary cells compared to normal mammary tissue, while weak immunostaining was associated with a diagnosis of carcinoma. Complementing earlier studies on IL-8 expression in several types of cancer, including mammary cancer, we conclude that IL-8 has potential for use as a prognostic marker for canine mammary neoplasia. (AU)