Association of IL1 gene polymorphisms with chronic periodontitis in Brazilians

Autor(es)

Trevilatto, Paula C. ^[1] ; de Souza Pardo, Ana Paula ^[2] ; Scarel-Caminaga, Raquel M. ^[3] ; de Brito, Jr., Rui B. ^[4] ; Alvim-Pereira, Fabiano ^[1] ; Alvim-Pereira, Claudia C. ^[1] ; Probst, Christian M. ^[5] ; Garlet, Gustavo P. ^[6] ; Sallum, Antonio W. ^[2] ; Line, Sergio R. P. ^[2]

Afiliação do(s) autor(es)

^[1] Pontifical Catholic Univ Parana PUCPR, Ctr Hlth & Biol Sci, BR-80215901 Curitiba, Parana - Brazil

^[2] Univ Estadual Campinas, Dent Sch Piracicaba, Dept Morphol, Piracicaba, SP - Brazil

^[3] Sao Paulo State Univ, UNESP, Sch Dent, Dept Diag & Surg, Araraquara, SP - Brazil

^[4] Sao Leopoldo Mand Dent Res Inst, Campinas, SP - Brazil

^[5] Fiocruz MS, Chagas Inst ICC, Curitiba, Parana - Brazil

^[6] Sao Paulo Univ FOB USP, Sch Dent Bauru, Dept Biol Sci, Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 56, n. 1, p. 54-62, JAN 2011.
• Citações Web of Science: 33

Resumo

Chronic periodontal disease (PD) is an infectious immune-inflammatory illness. Polymorphisms in IL1 genes play a role in inflammatory diseases through the modulation of cytokine levels. Objective: This study aimed to investigate the association between polymorphisms in the IL1 gene cluster and chronic periodontitis in a Brazilian population. Design: A sample of 113 subjects over 25 years (mean age 41.2) were grouped into: 44 healthy individuals, 31 subjects with moderate and 38 with severe periodontitis. DNA was obtained through a mouthwash and oral mucosa scraping. PCR-RFLP was used to identify the following polymorphisms: ILIA C - 889T (rs1800587), IL1B C - 511T (rs16944), IL1B C + 3954T (rs11436340), IL1RN intron 2 (rs2234663). Differences in the allele/genotype/haplotype frequencies were assessed by Chi-square test (p < 0.05). The risk associated with alleles, genotypes and haplotypes was calculated as odds ratio (OR) with 95% confidence intervals (CI). Results: Neither ILIA (C - 889T) nor IL1B (C + 3954T) polymorphisms was associated with chronic PD. Allele T for IL1B (C - 511T) only associated with PD in the group of blacks and mulattos. Moreover, genotype 2/2 for IL1RN (intron 2) was associated with severe PD. Conclusions: Genotype 2/2 of IL1RN for the whole Brazilian population and allele T of IL1B (C - 511T) in a subgroup of Afro-Americans and mulattos were suggested as putative risk indicators for chronic periodontitis. (C) 2010 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 99/05610-2 - Suscetibilidade genetica a severidade da doenca periodontal em adultos em familias com periodontite de inicio precoce.
• Beneficiário: Sergio Roberto Peres Line
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 99/06094-8 - Suscetibilidade genética a severidade da doença periodontal em adultos e em famílias com periodontite de início precoce
• Beneficiário: Paula Cristina Trevilatto
• Modalidade de apoio: Bolsas no Brasil - Doutorado