Proliferative, structural and molecular features of the Mdx mouse prostate

Autor(es)

Pinto, Leslie C. ^[1] ; Favaro, Wagner J. ^[1] ; Cagnon, Valeria H. A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Inst Biol, Dept Anat Cell Biol Physiol & Biophys, BR-13083865 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Experimental Pathology; v. 91, n. 5, p. 408-419, OCT 2010.
• Citações Web of Science: 2

Resumo

P>The prostate is fundamental to the male reproductive process, and the stroma-epithelium interaction has an important role in prostate maintenance. Studies suggest that dystroglycan (DG) plays a role in cancer development in various organs. Thus, the aims of this work were to characterize morphological and proliferative features of the prostatic stroma and epithelium of mdx mice; to verify the immunolocalization of the alpha and beta DG, IGF-1 and laminin alpha 3 receptors; and to relate those structural and molecular events to prostate pathogenesis and to verify the viability of this experimental model in prostate studies. Thirty male mice (mdx and C57BL10/Uni) were divided into control and mdx groups. Samples from the ventral prostate were collected for immunological, Western Blotting, transmission electron microscopy and morphometric analyses. Oestradiol and testosterone measurements were verified. The results showed diminished testosterone and increased oestradiol levels in the mdx group. Atrophied cells and hypertrophied stroma were seen in the mdx mice. Weak alpha and beta DG and laminin alpha 3 immunolocalization was demonstrated in the mdx group. Intense insulin-like growth factor receptor alpha-1 (IGFR alpha-1) localization was identified in the mdx animals. Thus, mdx animals showed changes in molecular and structural integrity and proliferation signals, leading to glandular homoeostasis imbalance, and compromise of prostate function. Also, the steroid hormone imbalance and the increased IGF-1 receptor level detected in mdx mice could be considered as a crucial factor in the pathogenesis of prostatic disorders. (AU)

• Processo FAPESP: 07/52053-0 - Perfis das distroglicanas e morfo-fisiologia do lobo ventral da próstata de camundongos distróficos
• Beneficiário: Leslie Cristina Pinto
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 07/54689-9 - Caracterização das distroglicanas e morfofisiologia do lobo ventral da próstata de camundongos distróficos (mdx)
• Beneficiário: Valéria Helena Alves Cagnon Quitete
• Modalidade de apoio: Auxílio à Pesquisa - Regular