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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Proliferative, structural and molecular features of the Mdx mouse prostate

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Author(s):
Pinto, Leslie C. [1] ; Favaro, Wagner J. [1] ; Cagnon, Valeria H. A. [1]
Total Authors: 3
Affiliation:
[1] Univ Campinas UNICAMP, Inst Biol, Dept Anat Cell Biol Physiol & Biophys, BR-13083865 Campinas, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: International Journal of Experimental Pathology; v. 91, n. 5, p. 408-419, OCT 2010.
Web of Science Citations: 2
Abstract

P>The prostate is fundamental to the male reproductive process, and the stroma-epithelium interaction has an important role in prostate maintenance. Studies suggest that dystroglycan (DG) plays a role in cancer development in various organs. Thus, the aims of this work were to characterize morphological and proliferative features of the prostatic stroma and epithelium of mdx mice; to verify the immunolocalization of the alpha and beta DG, IGF-1 and laminin alpha 3 receptors; and to relate those structural and molecular events to prostate pathogenesis and to verify the viability of this experimental model in prostate studies. Thirty male mice (mdx and C57BL10/Uni) were divided into control and mdx groups. Samples from the ventral prostate were collected for immunological, Western Blotting, transmission electron microscopy and morphometric analyses. Oestradiol and testosterone measurements were verified. The results showed diminished testosterone and increased oestradiol levels in the mdx group. Atrophied cells and hypertrophied stroma were seen in the mdx mice. Weak alpha and beta DG and laminin alpha 3 immunolocalization was demonstrated in the mdx group. Intense insulin-like growth factor receptor alpha-1 (IGFR alpha-1) localization was identified in the mdx animals. Thus, mdx animals showed changes in molecular and structural integrity and proliferation signals, leading to glandular homoeostasis imbalance, and compromise of prostate function. Also, the steroid hormone imbalance and the increased IGF-1 receptor level detected in mdx mice could be considered as a crucial factor in the pathogenesis of prostatic disorders. (AU)

FAPESP's process: 07/52053-0 - Dystroglycans and laminin 'BETA' 3 features on the prostate of mdx mice
Grantee:Leslie Cristina Pinto
Support Opportunities: Scholarships in Brazil - Master