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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Testosterone suppresses oxidative stress in human neutrophils

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Autor(es):
Marin, Douglas Popp [1] ; Bolin, Anaysa Paola [2] ; Macedo dos Santos, Rita de Cassia [2] ; Curi, Rui [3] ; Otton, Rosemari [2, 1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Cruzeiro do Sul Univ, Postgrad Program, Human Movement Sci Inst Phys Act & Sport Sci, BR-01506000 Sao Paulo - Brazil
[2] Cruzeiro do Sul Univ, BR-03342000 Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Phys & Biophys, BR-05508900 Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Cell Biochemistry and Function; v. 28, n. 5, p. 394-402, JUL 2010.
Citações Web of Science: 29
Resumo

The in vitro effect of testosterone on human neutrophil function was investigated. Blood neutrophils from healthy male subjects were isolated and treated with 10 nM, 0.1 and 10 mu M testosterone for 24 h. As compared with untreated cells, the testosterone treatment produced a significant decrease of superoxide production as indicated by the measurement of extra- and intracellular superoxide content. An increment in the production of nitric oxide was observed at 0.1 and 10 mu M testosterone concentrations, whereas no effect was found for 10 nM. Intracellular calcium mobilization was significantly increased at 10 nM, whereas it was reduced at 10 mu M testosterone. There was an increase in phagocytic capacity at 10 nM and a decrease of microbicidal activity in neutrophils treated with testosterone at 10 mu M. Glutathione reductase activity was increased by testosterone treatment, whereas no effect was observed in other antioxidant enzyme activities. An increase in the content of thiol groups was observed at all testosterone concentrations. Lipid peroxidation in neutrophils evaluated by levels of TBARS was decreased at 10 nM and 0.1 mu M testosterone. These results indicate the antioxidant properties of testosterone in neutrophils as suggested by reduction of superoxide anion production, and lipid peroxidation, and by the increase in nitric oxide production, glutathione reductase activity and the content of thiol groups. Therefore, the plasma levels of testosterone are important regulators of neutrophil function and so of the inflammatory response. Copyright (C) 2010 John Wiley \& Sons, Ltd. (AU)

Processo FAPESP: 07/03334-6 - Avaliação dos efeitos anti-oxidantes da astaxantina e seu papel regulador na funcionalidade de neutrófilos e linfócitos de diabéticos
Beneficiário:Rosemari Otton
Modalidade de apoio: Auxílio à Pesquisa - Regular