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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Testosterone suppresses oxidative stress in human neutrophils

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Author(s):
Marin, Douglas Popp [1] ; Bolin, Anaysa Paola [2] ; Macedo dos Santos, Rita de Cassia [2] ; Curi, Rui [3] ; Otton, Rosemari [2, 1]
Total Authors: 5
Affiliation:
[1] Cruzeiro do Sul Univ, Postgrad Program, Human Movement Sci Inst Phys Act & Sport Sci, BR-01506000 Sao Paulo - Brazil
[2] Cruzeiro do Sul Univ, BR-03342000 Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Phys & Biophys, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Cell Biochemistry and Function; v. 28, n. 5, p. 394-402, JUL 2010.
Web of Science Citations: 29
Abstract

The in vitro effect of testosterone on human neutrophil function was investigated. Blood neutrophils from healthy male subjects were isolated and treated with 10 nM, 0.1 and 10 mu M testosterone for 24 h. As compared with untreated cells, the testosterone treatment produced a significant decrease of superoxide production as indicated by the measurement of extra- and intracellular superoxide content. An increment in the production of nitric oxide was observed at 0.1 and 10 mu M testosterone concentrations, whereas no effect was found for 10 nM. Intracellular calcium mobilization was significantly increased at 10 nM, whereas it was reduced at 10 mu M testosterone. There was an increase in phagocytic capacity at 10 nM and a decrease of microbicidal activity in neutrophils treated with testosterone at 10 mu M. Glutathione reductase activity was increased by testosterone treatment, whereas no effect was observed in other antioxidant enzyme activities. An increase in the content of thiol groups was observed at all testosterone concentrations. Lipid peroxidation in neutrophils evaluated by levels of TBARS was decreased at 10 nM and 0.1 mu M testosterone. These results indicate the antioxidant properties of testosterone in neutrophils as suggested by reduction of superoxide anion production, and lipid peroxidation, and by the increase in nitric oxide production, glutathione reductase activity and the content of thiol groups. Therefore, the plasma levels of testosterone are important regulators of neutrophil function and so of the inflammatory response. Copyright (C) 2010 John Wiley \& Sons, Ltd. (AU)